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Test Code 0670 Tobramycin, Peak, Plasma

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Enzyme Immunoassay

Testing Algorithm:

If tobramycin value is a critical result , #1883 Potassium, Plasma will be performed, at an additional charge.

Literature References:

Package insert: Tobramycin TOBR2. Roche Diagnostics, 02/2015.

Specimen Requirements

Specimen Type: Plasma
Container/Tube: Mint-green top (lithium heparin)
Specimen Volume: Full tube

 

Collection Instructions: Draw blood 60 to 90 minutes after completion of drug infusion for intravenous dose.

Additional Information: Date and time of draw are required.

Day(s) Test Set Up

Monday through Sunday
Turnaround Time

STAT:  1 hour after receipt in laboratory

Routine:  2-4 hours after receipt in laboratory 

 

Reference Values

Setting Target Range
Unspecified indication 3-10 mcg/mL
UTI/GPC synergy* 3-5 mcg/mL
Systemic illness 6-8 mcg/mL
Sepsis 8-10 mcg/mL
Physician alert value (automatic call-back)

≥21 mcg/mL

*UTI/GPC synergy refers to the synergistic effect of aminoglycosides with other antibiotics in treating urinary tract infections caused by gram-positive cocci (eg, Enterococcus).

Note: See Dosage Adjustment of Aminoglycoside Antibiotics Based on Drug Level Monitoring in Special Instructions.

Literature Reference:

Casabar E, BJH Antibiotic Utilization Review Subcommittee. Aminoglycoside dosing: Extended interval and traditional. BJH Pharmacy’s Toolbook (2005).

Test Classification and CPT Coding

80200-Tobramycin

84132-Potassium (if appropriate)

Additional Information

For BJH Laboratory Use Only

Alternate Tube(s):

1 dark green-top (lithium or sodium heparin) tube

1 red-top tube

1 lavender-top (EDTA) tube

Separator gel tube is acceptable.

Analyte Stability:

Specimen should be tested within 8 hours of collection if kept at ambient temperature. If specimen must be stored for later testing; it may be kept at 2° C to 8° C for up to 24 hours, or at ≤-20° C for longer periods. Specimens containing additional antibiotics should be stored frozen if a delay in analysis of more than 8 hours is anticipated. Failure to freeze specimens containing additional antibiotics may result in falsely-low tobramycin levels due to in vitro inactivation.

Laboratory Processing Instructions:

Test performed in BJH Core Lab.  Processed by automated line for delivery to the Cobas 8000. Completed specimens are stored refrigerated for 5 days.

Specimen Transport Temperature

Ambient

LOINC Code Information

Name LOINC Code
Tobramycin, Peak, Plasma 4057-6