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Test Code 3125 Urine Macroscopic

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Note: The preferred test for routine urinalysis is #8705 Urine Reflex (Urine Macroscopic with Reflex Microscopic, if Indicated).

Method Name:

Dipstick: Spectrophotometric reflectance of light on color reagent pads

Urine color: Spectrophotometry or visual observation

Specific gravity: Refractometry

Clarity: Measurement of the transmission and scatter of light

Includes color, clarity, specific gravity, pH, protein, glucose, ketones, bilirubin, occult blood, urobilinogen, nitrites, and leukocyte esterase.

Literature References:

1. Henry JB, et al. Clinical diagnosis and management by laboratory methods. 20th ed. Philadelphia, PA: Saunders, 2001:371-85.
2. Package insert: AUTION sticks 9EB for urine chemistry, ARKRAY, Inc., Rev.2/2005.

Specimen Requirements

Specimen Type: Urine, random
Container/Tube: Yellow-top, conical urine collection tube

Specimen Volume: Full tube

Specimen Minimum Volume: 10 mL (3 to 8 mL specimen will be analyzed, however results may be modified by comment “<8 mL of urine received; normal range is based on 8 mL”)

Collection Instructions:

1. No additive.

2. Collect a midstream voided specimen following directions printed on outer wrapping of Urine Complete Kit.
3. Using cannula in collection cup lid, fill yellow-top, plastic, conical tube.

Additional Information:

1. Label tube with patient’s name and date of birth.

2. A catheterized specimen is acceptable.
3. Provide collection date and time, and method of collection.

4. If storage and transport is >1 hour, refrigerate specimen.

5. Specimen should be received in the laboratory within 1 hour of collection. Specimen >12 hours old will not be tested except for non-hospital specimen having physician approval.

Day(s) Test Set Up

Monday through Sunday

Turnaround Time (STAT, Routine)   

STAT: 1 hour after receipt in laboratory

Routine: 2-4 hours after receipt in laboratory

Reference Values

Specific gravity: 1.003-1.030
pH: 5.0-8.0
Protein: negative-trace
Glucose, qualitative: negative
Ketones, qualitative: negative
Bilirubin, qualitative: negative
Occult blood: negative
Urobilinogen: <2.0 mg/dL
Nitrites: negative
Leukocyte esterase: negative

Literature References:

1. Henry JB, et al. Clinical diagnosis and management by laboratory methods. 20th ed. Philadelphia, PA: Saunders, 2001:1432-34.
2. Brunzel NA. Fundamentals of urine and body fluid analysis. 2nd ed. Philadelphia, PA: Saunders, 2004:419.

Test Classification and CPT Coding

81003-Urine macroscopic

81005-Urine qualitative/semi quantitative (if appropriate)

Additional Information

For BJH Laboratory Use Only

Alternate Tube(s):

1 non-sterile, plastic urine container

1 plain, red top tube

Analyte Stability:

Specimen should be received in the laboratory within 1 hour of collection. Specimen >12 hours old will not be tested, except for non-hospital specimen having physician approval.

Laboratory Processing Instructions:

Test performed in BJH Hematology. Performed on uncentrifuged urine.

Specimen Transport Temperature

Ambient <1 hour/Refrigerate >1 hour OK

LOINC Code Information

Name LOINC code
Color 50553-7
Clarity 32167-9
pH Ur Ql 5803-2
Specific Gravity Ur 50562-8
Albumin Ur Ql 20454-5
Blood Ur Ql 5794-3
Glucose Ur Ql 25428-4
Ketones Ur Ql 2514-8
Leuk Esterase Ur 5799-2
Nitrite Ur Ql 32710-6
Urobilinogen Ur Ql 20405-7