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Test Code 3208 Hemoglobin Analysis, Blood

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Capillary Electrophoresis/Gel Electrophoresis

Testing Algorithm:

On all first time patients, for hemoglobins that appear in the S or C position, confirmatory testing by acid gel electrophoresis will be performed, at an additional charge. For all other hemoglobins, confirmation testing by acid and alkaline gel electrophoresis will be performed, at an additional charge.

Specimen Requirements

Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: Full tube

 

Day(s) Test Set Up

Tuesday and Thursday

Turnaround Time:
STAT: not available
Routine: Normal findings are resulted Tuesday and Thursday; abnormal finding confirmation results available once signed by medical director-normally by 1530 Friday.

Reference Values

Hemoglobin A1: 96.0%-98.5%
Hemoglobin A2: 1.5%-3.2%

Hemoglobin F: ≤1.0%

Literature References:

1. Hoyer JD, Kroft SH. Color atlas hemoglobin disorders:  a compendium based on proficiency testing. College of American Pathologist, 2003.

2. Giordano, PC. Carrier diagnostics and prevention of hemoglobinopathies using capillary electrophoresis. Sebia Laboratory, 2007.

Test Classification and CPT Coding

83020-Capillary electrophoresis

83020-Acid hemoglobin electrophoresis (if appropriate)

83020-Alkaline hemoglobin electrophoresis (if appropriate)

Additional Information

For BJH Laboratory Use Only
Analyte Stability:

7 days refrigerated

Laboratory Processing Instructions:

Test performed in BJH Immunology. If volume is 2 mL, place tube on the Coulter automated line for RBC profile. If <2 mL, run in the secondary mode. Refrigerate specimen until assay.

Specimen Transport Temperature

Ambient

LOINC Code Information

Name LOINC Code
Acid Hemoglobin Electrophoresis 13515-2
Alkaline Hemoglobin Electrophoresis 49323-9
Hemoglobin A 20572-4
Hemoglobin A2 27345-8
Hemoglobin C 32681-9
Hemoglobin F 32682-7
Hemoglobin S 32683-5
Hemoglobin Analysis Interpretation 20492-5