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Test Code 4390 Factor VIII Inhibitor, Quantitative, Plasma

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Absorbance measurement, Clot signature formation 

Literature References:

HemosIL SynthASil (pn 20006800) package insert issued 02/2016, Instrumentation Laboratory.

Specimen Requirements

Specimen Type: Plasma

Container/Tube: Light-blue top (citrate)

Specimen Volume: 4 Full tubes

Specimen Minimum Volume: 4 Full tubes

Collection Instructions: 

1. Draw a discard tube prior to light-blue top (citrate) tube.
2. Tube should remain stoppered.
3. Do not centrifuge.
4. Clotted blood or an insufficient specimen is not acceptable.
5. Avoid hemolysis and heparin contamination of specimen.
Analyte Stability:
4 hours at 18° C to 24° C if the top has not been removed.
2 hours at 18° C to 24° C after the top has been removed.
Platelet-free plasma can be stored 6 months at -70° C.
Additional Information: If the patient is a hospital patient and the hematocrit is >55%, call Barnes-Jewish Hematology Laboratory at 314-362-5203 for instructions.

Day(s) Test Set Up

Monday, Thursday

Cut-off time: 0700

Turnaround Time:
STAT: not available
Routine: results available the day of testing

Reference Values

<0.5 Bethesda Units

Literature References:

Kasper CK. A more uniform measurement of factor VIII inhibitors. Throm Diath Haemorrh 1975;34:86972.

Test Classification and CPT Coding

85335

Additional Information

For BJH Laboratory Use Only
Analyte Stability:

4 hours at 18° C to 24° C if the top has not been removed.
2 hours at 18° C to 24° C after the top has been removed.
Platelet-free plasma can be stored 6 months at -70° C.

Specimen cannot be frozen or refrigerated.

Laboratory Processing Instructions:

Test performed in BJH Hematology.

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen Yes

Ambient Whole Blood:  4 hours at 18° C to 24° C

Frozen Platelet-Free Plasma: 6 months at -70° C

LOINC Code Information

Name LOINC Code
Factor VIII Inhibitor, Quantitative, Plasma   3196-3