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Test Code 4605 ABPA Cascade

Performing Laboratory

St. Louis Children's Hospital and Mayo Medical Laboratories

Last Reviewed

January 2016

Testing Algorithm

If Immunoglobulin E (IgE) is ≥417.00 IUnits/mL, then Aspergillus fumigatus (M3) IgE Antibodies is performed at an additional charge.

If Aspergillus fumigatus IgE is positive (>0.35 kUnits/L), then Aspergillus fumigatus IgG Antibodies will be sent to outside reference lab at an additional charge.

Interpretive

IgE Total
<1 week: None established

1 week-4 weeks: 0.30-1.00 IUnits/mL
4 weeks-26 weeks: 0.80-12.00 IUnits/mL

26 weeks-1 year: 0.50-11.80 IUnits/mL
1 year-2 years: 1.30-28.70 IUnits/mL

2 years-4 years: 2.10-33.00 IUnits/mL
4 years-6 years: 7.90-55.00 IUnits/mL

6 years-8 years: 2.20-87.80 IUnits/mL
8 years-12 years: 3.10-110.80 IUnits/mL

12 years-18 years: 6.10-96.50 IUnits/mL
>18 years: 0.00-250.00 IUnits/mL

 

Aspergillus fumigatus IgE (M3)

 

Class IgE kU/L Interpretation
0 <0.35 Negative
1 0.35-0.69 Equivocal
2 0.70-3.49 Positive
3 3.50-17.4 Positive
4 17.5-49.9 Strongly positive
5 50.0-99.9 Strongly positive
6 ≥100 Strongly positive


Aspergillus fumigatus IgG
<4 years: None established

≥ 4 years: ≤ 102 mg/L

Critical Values: NA

Limitations: NA

 

Methodology:

IgE Total: Electrochemiluminescence immunoassay (ECLIA) sandwich principle
Asper fum IgG: Fluorescence Enzyme Immunoassay (FEIA)

Specimen Requirements

Specimen Type: Blood

Container/Tube

Preferred: Gold/Red SST Vacutainer®

Acceptable: Plain red-top vacutainer ®

Collection Volume: 3.0 mL (minimum 1.5 mL)

Patient Preparation: None

Sample Rejection: Mislabeled or unlabeled specimens.

Special Instructions: Send to lab promptly.

Logistics

Test Availability:

IgE Total: Monday-Sunday

Aspergillus fum, IgE specific: Monday-Friday

Aspergillus fum, IgG: Monday-Saturday
 

Cut-off time:
IgE Total: None; performed as received

 

Turnaround Time:

IgE Total: Same day; 2 hours from receipt in lab

If reflex testing required: 2-6 days

 

Lab Testing Section: Chemistry

Reflex testing done in Immuno/Sero and/or Mayo Medical Laboratory if required.

Lab Dept

Core Lab

Additional Information

For SLCH Laboratory use only

 

Lab Processing instructions: Centrifuge specimen to separate. Split the specimen by aliquoting 0.5 mL of specimen into a conical tube for the CAP250 and then giving the SST collection tube to the Chemistry bench for analysis.

 

Processed Volume: 0.8 mL plasma/serum

 

Analyte Stability:
Ambient: 8 hours

Refrigerated: 7 days

Frozen: 6 months

 

Notes: If Asper fum IgG is required, send a minimum of 0.3 mL of serum to Mayo, refrigerated.

General Information

KIDDOS order name: Allergic Bronchopulmonary Aspergillosis Cascade
Cerner order name: ABPA Cascade (SLCH)

CPT Codes:

82785 - IgE

86003 - Aspergillus fum IgE (if appropriate)

86606 - Aspergillus fum IgG (if appropriate)
 

LOINC Value:
IgE Total: 19133-0

Asper fum IgE: 6025-1

Asper fum IgG: 26954-8

Synonyms:

Barnes-Jewish Hospital Additional Information:
For BJH Laboratory Use Only

Laboratory Processing Instructions:
BJH Hematology will forward to the performing laboratory.
Reflex Testing:
#4607 Aspergillus fumigatus, IgE Specific (M3), Serum is performed at St. Louis Children’s Hospital (SLCH).
#4608 Aspergillus fumigatus, IgG Antibodies, Serum is performed at Mayo Medical Laboratories, forwarded by SLCH.