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Test Code 5065 Immune Competence Assessment

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Profile Information:
CD3 CD8 Absolute
CD3 Absolute CD19
CD4 CD19 Absolute
CD4 Absolute CD16+CD56
CD4/CD8 Ratio CD16+CD56 Absolute
CD8  

Method Name:

Flow Cytometry-Single Platform

Literature References:

1. U.S. Department of Health and Human Services, Public Health Service, CDC. Flow cytometric immunophenotyping quality assurance and quality procedures for CD4+T-lymphocyte determinations. 04/1993.
2. NCCLS tentative guidelines. Clinical application of flow cytometry: quality assurance and immunophenotyping of peripheral blood lymphocytes. H42T 12:6.

3. MMWR Recommendation and Reports, Guidelines for performing single-platform absolute CD4 determination with CD45 gating for persons infected human immunodeficiency virus, January 21, 2003.

Specimen Requirements

Specimen must arrive within 24 hours of draw. Specimen must arrive by 1600 on Friday and 1 day before a holiday.

 

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: Full tube

Specimen Minimum Volume: 1 mL

Additional Information: Specimen cannot be refrigerated.

Day(s) Test Set Up

Monday through Friday

Cut-off time: 2200 Monday through Thursday; 1600 Friday and eve before a holiday

Turnaround Time:
STAT: not available
Routine: 48 hours

Reference Values

CD3 (PAN T-CELLS)

Percentage: 57%-85%
Absolute: 706-2,482
CD4 (HELPER/INDUCER T-CELLS)

Percentage: 27%-58%
Absolute: 393-1,607

CD4/CD8 RATIO

0.8-3.8
CD8 (CYTOTOXIC/SUPPRESSOR T-CELLS)

Percentage: 14%-42%
Absolute: 211-977
CD16+CD56 (NATURAL KILLER CELLS)

Percentage: 6%-32%
Absolute: 105-710

CD19 (B-CELLS)

Percentage: 4%-22%
Absolute: 79-545

Test Classification and CPT Coding

86355-B-cells, total count

86357-Natural killer cells, total count

86359-T-cells, total count

86360-T-cells CD4+CD8 with ratio

Additional Information

For BJH Laboratory Use Only
Alternate Tube:

1 dark green-top (sodium heparin) tube

Analyte Stability:

Specimen cannot be refrigerated. Specimen must be processed within 48 hours of draw and kept at ambient temperature.

Laboratory Processing Instructions:
Test performed in BJH Flow Cytometry.

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen NO