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Test Code 5085 Immunofixation Electrophoresis, Urine

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Gel Electrophoresis/Immunoprecipitation

Note: This test is automatically performed when #5309 Protein Electrophoresis, 24 Hour, Urine is ordered.

Literature Reference:

Whicher JT, Hawkins L, Higginson J. Clinical applications of immunofixation: a more sensitive technique for the detection of Bence-Jones protein. J Clin Pathol 988;33:779-80.

Specimen Requirements

Submit only 1 of the following specimens:

 

Preferred

Specimen Type: Urine, 24 hour

Container/Tube: Plain brown urine container

Specimen Volume: Entire collection

Collection Instructions: No additive. Refrigerate specimen during collection.

Additional Information:

1. Starting and ending times of collection are required.

2. See Urine Collection in Special Instructions for additional information.

3. Collection periods <20 hours will be ordered as a timed-urine collection.

4. Collection periods >48 hours require approval from the Laboratory Medicine resident before analysis.

 

Specimen Type: Urine, random

Container/Tube: Urine Complete Cup Kit

Specimen Volume: 20 mL from a first-morning collection.

Collection Instructions: No additive.

Additional Information: See Urine Collection in Special Instructions for additional information.

 

Alternate

Specimen Type: Urine, timed collection

Container/Tube: Plain brown urine container

Specimen Volume: Entire collection

Collection Instructions: No additive. Refrigerate specimen during collection.

Additional Information:

1. Starting and ending times of collection are required.

2. See Urine Collection in Special Instructions for additional information.

3. Collection periods <20 hours will be ordered as a timed-urine collection.

4. Collection periods >48 hours require approval from the Laboratory Medicine resident before analysis.

Day(s) Test Set Up

Monday through Friday

Cut-off time: 0800
Turnaround Time:
STAT: not available
Routine: 2 working days

Reference Values

No monoclonal protein detected.

Literature Reference:

Alper CA, Johnson AM. Immunofixation electrophoresis: a technique for the study of protein polymorphism. Vox Sang 1969;17:445-52.

Test Classification and CPT Coding

86335

Additional Information

For BJH Laboratory Use Only
Alternate Tube:

Plain, brown urine container preferred; any clean container will be accepted.

Analyte Stability:

24 hours at ambient temperature is acceptable; refrigeration during collection is preferred.

Laboratory Processing Instructions:

Test performed in BJH Immunology. If ordered with a urine protein electrophoresis; this is a duplicate and should be cancelled. If ordered alone; do not send an aliquot to Chemistry for total protein, and concentrate 100x. Keep a 10-mL urine specimen in refrigerator for possible future use. Place concentrate in appropriate rack.

Specimen Transport Temperature

Ambient

Special Instructions

LOINC Code Information

Name LOINC Code
Immunofixation, Urine Interpretation 14896-5