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Test Code 5332 Leukemia/Lymphoma Cell Marker Assessment

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Flow Cytometry

Specimen Requirements

Specimen must arrive as soon as possible after collection. Specimen must arrive by 2000 on Friday and 1 day before a holiday.

 

Forms: Flow Cytometry Immunophenotyping Request and a Barnes-Jewish Hospital Request Form in Special Instructions.

 

Specimen Type: Bone marrow

Container/Tube: Dark-green top (sodium heparin)

Specimen Volume: 1-5 mL

Additional Information:

1. Collection date is required.

2. A pathology/diagnostic report, the name and telephone number of the ordering physician, and a brief history are essential to achieve a consultation fully relevant to the ordering physician’s needs.

3. Specimen cannot be frozen.

4. Label specimen appropriately (bone marrow).

 

Specimen Type: CSF

Container/Tube: Sterile container

Specimen Volume: Volume of fluid necessary to phenotype depends on the cell count of the specimen

Additional Information:

1. Collection date is required.

2. A pathology/diagnostic report, the name and telephone number of the ordering physician, and a brief history are essential to achieve a consultation fully relevant to the ordering physician’s needs.

3. Specimen cannot be frozen.

4. Label specimen appropriately (CSF).

 

Specimen Type: Fluid, miscellaneous

Container/Tube: Sterile container

Specimen Volume: 10-20 mL

Additional Information:

1. Collection date is required.

2. A pathology/diagnostic report, the name and telephone number of the ordering physician, and a brief history are essential to achieve a consultation fully relevant to the ordering physician’s needs.

3. Specimen cannot be frozen.

4. Label specimen appropriately (fluid).

 

Specimen Type: Lymph node

Container/Tube: Screw-capped container with RPMI

Specimen Volume: 1 cm3 biopsy

Additional Information:

1. Collection date is required.

2. A pathology/diagnostic report, the name and telephone number of the ordering physician, and a brief history are essential to achieve a consultation fully relevant to the ordering physician’s needs.

3. Specimen cannot be frozen.

4. Label specimen appropriately (lymph node).

 

Specimen Type: Whole blood

Container/Tube: 2 dark-green top (sodium heparin) tubes and 1 lavender top (EDTA) tube

Specimen Volume: 5 mL of sodium heparin whole blood in each tube and 2 mL of EDTA whole blood

Collection Instructions: Do not transfer blood to other containers.

Additional Information:

1. Collection date is required.

2. A pathology/diagnostic report, the name and telephone number of the ordering physician, and a brief history are essential to achieve a consultation fully relevant to the ordering physician’s needs.

3. Specimen cannot be frozen.

4. Label specimen appropriately (blood).

Day(s) Test Set Up

Monday through Friday, excluding holidays

Reference Values

The pathologist will select the appropriate antibody panel for testing based on clinical information and morphologic review. Report is issued through surgical pathology.

Test Classification and CPT Coding

88184-Flow cytometry, first marker
88185-Flow cytometry, additional markers

88313-Special stain group 2 (if appropriate)
88319 x 2-Determinative histochemistry (if appropriate)
Note: Professional charges may be applied.

Additional Information

For BJH Laboratory Use Only
Laboratory Processing Instructions:

Test performed in BJH Flow Cytometry. Verbal preliminary results may be obtained by paging the hematopathology fellow at 314-836-4742.

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen NO