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Test Code 5510 Paroxysmal Nocturnal Hemoglobinuria (PNH) Profile

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Flow Cytometry

Includes CD59-RBC membrane inhibitor of reactive lysis (MIRL), CD59-WBC MIRL, and fluorescent-labeled inactive toxin aerolysis (FLAER).

Literature References:

1. Krauss JS. Laboratory diagnosis of paroxysmal nocturnal hemoglobinuria. annals of clinical and laboratory science 2003;33:401-6.
2. Pui JC, Schreitzenmeir R, Moore, JS. Flow cytometric diagnosis of paroxysmal nocturnal hemoglobinuria, Purdue Education Flow Vol 3.

Specimen Requirements

Specimen must arrive within 48 hours of draw. Specimen must arrive by 1800 on Friday and 1 day before a holiday.

 

EDTA and sodium heparin whole blood are required.

 

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: Full tube

Specimen Minimum Volume: 1 mL or 5 mL if 1 tube is submitted (Testing can be performed on either EDTA or sodium heparin whole blood if only 1 tube with is submitted with sufficient volume.)

 

Specimen Type: Whole blood

Container/Tube: 10-mL dark-green top (sodium heparin)

Specimen Volume: Full tube

Specimen Minimum Volume: 5 mL or 5 mL if 1 tube is submitted (Testing can be performed on either EDTA or sodium heparin whole blood if only 1 tube with is submitted with sufficient volume.)

Day(s) Test Set Up

Monday through Friday

Cut-off time: 1800

Turnaround Time:
STAT: not available
Routine: results available within 48 hours.

Reference Values

CD59-RBC: >95%
CD59-WBC: >80%
FLAER: 100%
Above percentages are guidelines, the final interpretation will be determined by the hematopathologist. A surgical pathology report will be provided.

Test Classification and CPT Coding

88184-Flow cytometry, first marker
88185 x 2-Flow cytometry, additional markers

Additional professional charges may apply.

Additional Information

For BJH Laboratory Use Only

Alternate Tube:

1 dark green-top (lithium heparin) tube

1 unspun, mint green-top (lithium heparin) tube

Analyte Stability:

CD59-RBC is stable for 1 week if specimen is refrigerated.

FLAER is stable for 4 days if specimen is refrigerated.

CD59-WBC is stable for 48 hours if specimen is refrigerated.

Laboratory Processing Instructions:

Test performed in BJH Flow Cytometry. 6 wedge smears air-dried and stained.

Specimen Transport Temperature

Ambient