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Test Code 5515 Immunoglobulin Heavy-Chain (IGH) Hypermutation (IGH V Region) Sequencing

Performing Laboratory

Barnes-Jewish Hospital Laboratory


Polymerase Chain Reaction (PCR)/Sequencing Analysis
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Literature References:

1. Miller, JE, Wilson, SS, Jaye, DL, et al. Molecular Diagnostics, 1999:4;101-7.

2. Package insert: InVivo Scribe Technologies: IGH gene clonality assay, 2004.

Specimen Requirements

Call Barnes-Jewish Molecular Diagnostics Laboratory at 314-454-8685 for collection instructions.


Forms: Molecular Diagnostics Laboratory Request for DNA Studies-Oncology in Special Instructions.


Submit only 1 of the following specimens:


Specimen Type: Blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: Full tube

Specimen Minimum Volume: 1 to 2 mL

Collection Instructions:

1. Invert several times to mix blood. Clotted blood is not acceptable.

2. Specimen cannot be frozen.


Specimen Type: Separated cell pellets

Container/Tube: Sterile, plastic container

Specimen Volume: 1 x 106 nucleated cells

Additional Information: Forward promptly on dry ice.


Specimen Type: Tissue, formalin-fixed, paraffin-embedded

Container/Tube: Sterile, plastic microcentrifuge tube

Specimen Volume: Twenty, 10-micron sections of tissue


Specimen Type: Tissue, fresh

Container/Tube: Sterile, plastic container

Specimen Volume: 10 mm3

Additional Information: Forward frozen tissue on dry ice.

Day(s) Test Set Up


Turnaround Time:
STAT: not available
Routine: 5 to 7 business days

Reference Values

An interpretive report will be provided.

Literature References:

1. Damle RN, Wasil T, Fais F, et al. Ig V gene mutation status and CD38 expression as novel prognostic indicators in chronic lymphocytic leukemia. Blood 1999;94:1840-7.

2. Miller, JE, Wilson, SS, Jaye, DL, et al. Molecular diagnostics, 1999:4;101-7.

Test Classification and CPT Coding


Additional Information

For BJH Laboratory Use Only
Analyte Stability:

Blood is stable 0 to 4 hours at ambient temperature, 96 hours at 4° C.
FFPE tissue stable indefinitely.
Cell pellets and fresh-frozen tissue are stable at -20° C.

Laboratory Processing Instructions:
Test performed in BJH Molecular Diagnostics.

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen NO-Blood; Tissue, Formalin-Fixed, Paraffin-Embedded

Frozen on Dry Ice-Tissue, fresh; Separated Cell Pellets