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Test Code 5856 Immunoglobulin Heavy-Chain (IGH) Gene Rearrangement

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Polymerase chain reaction (PCR) amplification of the conserved framework (FR) and joining (J) regions of the Immunoglobulin Heavy Chain gene followed by capillary electrophoresis for size separation.

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Literature Reference:

vanDongen JJL, Langerak AW, Bruggemann M, et al.  Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and t-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 concerted Action BMH4-CT98-3936. Leukemia 2003;17:2257-2317.

Specimen Requirements

Call Barnes-Jewish Hospital Molecular Diagnostics Laboratory at 314-454-8685 for collection instructions.

 

Forms: Molecular Diagnostics Laboratory Request for DNA Studies-Oncology in Special Instructions.

 

Submit only 1 of the following specimens:

 

Specimen Type: Blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: Full tube

Specimen Minimum Volume: 1 to 2 mL

Collection Instructions:

1. Invert several times to mix blood.Clotted blood is not acceptable.

2. Specimen cannot be frozen.

 

Specimen Type: Bone marrow

Container/Tube: Lavender top (EDTA)

Specimen Volume: 1 to 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow. Clotted bone marrow is not acceptable.

2. Specimen cannot be frozen.

 

Specimen Type: Tissue, formalin-fixed, paraffin-embedded

Container/Tube: Sterile, microcentrifuge tube

Specimen Volume: Twenty, 10-micron sections

 

Specimen Type: Tissue, fresh

Container/Tube: Sterile, plastic container

Specimen Volume: 10 mm3

Additional Information: Forward frozen tissue on dry ice.

Day(s) Test Set Up

Monday 
Turnaround Time:
STAT: not available
Routine: 7 to 10 business days

Reference Values

An interpretative report will be provided.

Literature Reference:

vanDongen JJL, Langerak AW, Bruggemann M, et al.  Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and t-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 concerted Action BMH4-CT98-3936. Leukemia 2003;17:2257-2317.

Test Classification and CPT Coding

81261 

Additional Information

For BJH Laboratory Use Only
Alternate Tube:

1 yellow-top ACD (solution A or B) tube

Analyte Stability:

Blood and bone marrow are stable 0 to 4 hours at ambient temperature, up to 96 hours refrigerated.
FFPE tissue stable indefinitely.
Fresh-frozen tissue is stable at -20° C.

Laboratory Processing Instructions:
Test performed in BJH Molecular Diagnostics.

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen NO-Blood; Bone Marrow; Tissue, Formalin-Fixed, Paraffin-Embedded

Frozen on Dry Ice-Tissue, Fresh