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Test Code 5952 T Cell Receptor Gamma (TCRG) Gene Rearrangement

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

(TCRG) variable (V) and joining (J) regions that flank the hypervariable antigen-binding region 3 (CDR3) followed by capillary electrophoresis for size separation.
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)
Literature Reference:
vanDongen JJL, Langerak AW, Bruggemann M, et al.  Design and standardization of PCR primers and Polymerase Chain Reaction (PCR) amplification of conserved regions within the T Cell Receptor Gamma protocols for detection of clonal immunoglobulin and t-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 concerted Action BMH4-CT98-3936. Leukemia 2003;17:2257-2317.

Specimen Requirements

Call Barnes-Jewish Hospital Molecular Diagnostics Laboratory at 314-454-8685 for collection instructions.
 
Forms: Molecular Diagnostics Laboratory Request for DNA Studies-Oncology in Special Instructions.
 
Submit only 1 of the following specimens:
 
Specimen Type: Blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: Full tube
Specimen Minimum Volume: 1 to 2 mL
Collection Instructions:
1. Invert several times to mix blood.Clotted blood is not acceptable.
2. Specimen cannot be frozen.
 
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA)
Specimen Volume: 1 to 2 mL
Collection Instructions:
1. Invert several times to mix bone marrow. Clotted bone marrow is not acceptable.
2. Specimen cannot be frozen.
 
Specimen Type: Tissue, formalin-fixed, paraffin-embedded
Container/Tube: Sterile, microcentrifuge tube
Specimen Volume: Twenty, 10-micron sections
 
Specimen Type: Tissue, fresh
Container/Tube: Sterile, plastic container
Specimen Volume: 10 mm3
Additional Information: Forward frozen tissue on dry ice.

Day(s) Test Set Up

Monday
Turnaround Time:
STAT: Director approval required
Routine: 7 to 10 business days

Reference Values

An interpretative report will be provided.

Test Classification and CPT Coding

81342 

Additional Information


For BJH Laboratory Use Only
Alternate Tube:
1 yellow-top ACD (solution A or B) tube
Analyte Stability:
Blood and bone marrow are stable 0 to 4 hours at ambient temperature, up to 96 hours refrigerated.
FFPE tissue stable indefinitely.
Fresh-frozen tissue is stable at -20° C.

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen NO-Blood; Bone Marrow; Tissue, Formalin-Fixed, Paraffin-Embedded
Frozen on Dry Ice-Tissue, Fresh