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Test Code 6100 Endomysial Antibodies (IgA), Serum

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Indirect Immunofluorescence Assay

Testing Algorithm:

If endomysial antibody screen is positive, endomysial antibody titer will be performed at an additional charge.

Literature Reference:

Vitoria JC, Arrieta A, Arranz C, et al. Antibodies to gliadin, endomysium, and tissue transglutaminase for the diagnosis of celiac disease. J Pediatr Gastroenterol Nutr 1999;29:571-4.

Specimen Requirements

Specimen Type: Serum

Container/Tube: Plain red top

Specimen Volume: Full tube

 

Day(s) Test Set Up

Tuesday, Friday

Cut-off time: 0800

Turnaround Time:
STAT: not available
Routine: results available the day of testing

Reference Values

Negative in normal individuals and in dermatitis herpetiformis or celiac disease patients on gluten-free diet. Report includes presence and titer of circulating antibody.

Literature Reference:

Binder WL, Gustoafson D, Martis P, et al. High performance anti-gliadin antibody assay: use of defined synthetic peptides. [poster]. INOVA Diagnostics.

Test Classification and CPT Coding

86255-Fluorescent antibody screen
86256-Fluorescent antibody titer (if appropriate)

Additional Information

For BJH Laboratory Use Only

Alternate Tube:

SST Serum gel tube
Analyte Stability:

Serum stable indefinitely at -20° C

Laboratory Processing Instructions:

Test performed in BJH Immunology. Separate serum from clot as soon as possible.

Specimen Transport Temperature

Ambient

LOINC Code Information

Name LOINC Code
Endomysial Antibody, Qual 16814-6
Endomysial Antibody, IgA, Quant 25399-7