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Test Code 76480001 Obstetric Panel with HIV

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Profile Information:
Complete Blood Count (CBC) Rubella Antibodies, IgG
Hepatitis Bs Antigen (HBsAg) Type and Screen
Rapid Plasma Reagin (RPR), Qualitative HIV-1/HIV-2 Ab + p24 Ag

Method Name:

See individual test listings.

Testing Algorithm:

If HBsAg is positive, then hepatitis Bs antigen confirmation will be performed, at an additional charge. If antibody screen is positive, then antibody identification and #9874 Antibody Titration, Blood may be performed, at an additional charge. 

If HIV reactive, antibody differentiation testing is performed.  If the differentiation test is reactive for HIV-1 antibodies only, or reactive for HIV-1 and HIV-2 antibodies, or is not reactive for HIV-1 and HIV-2 antibodies.  HIV-1 RNA (viral load) test is recommended  to be performed with a separate specimen at BJH Molecular Diagnostics Laboratory.  If the differentiation test is reactive for HIV-2 antibodies, HIV-2 PCR can be considered with the assistance of the LMR, since HIV-2 infection is rare in the United States.  The patient's history and clinical condition can be considered if HIV-2 PCR testing is required.

Specimen Requirements

2 ETDA tubes, 1 Pink top, 1 Mint green tube,  and 1 Plain red top tube are required.

 

Hospital Patient

CBC

Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)-Pink-top (EDTA) tube is not acceptable.

Specimen Volume: Full tube

 

Collection Instructions: Immediately invert several times to mix blood.

Additional Information: Label specimen appropriately (blood for CBC).

 

Type and Screen

Specimen Type: Whole blood
Container/Tube: Pink top (EDTA)-Separator gel tube is not acceptable.

Specimen Volume: Full tube

 

Additional Information:

1. Specimen must be labeled with barcode label or addressograph imprint including patient’s name, hospital registration number, and date of birth.
2. Specimen must have 2 signatures (phlebotomist and witness). Initials are not acceptable.

 

HBsAg

Specimen Type: Serum
Container/Tube: Plain red top
Specimen Volume: Full tube

 

Additional Information: Label specimen appropriately 

 

Rubella

Specimen Type:  Plasma

Container/Tube:  Mint green tube

Specimen Volume:  Full tube

Additional Information:  label specimen appropriately

 

RPR

Specimen Type: Serum
Container/Tube: Plain red top
Specimen Volume: Full tube

 

Additional Information: Label specimen appropriately (serum for RPR).

 

HIV-1/HIV-2 Ab +p24 Ag

Specimen Type:  Plasma

Container/Tube:  Pink top tube

Specimen Volume:  Full tube (7mL)

 

 

 

 

Non Hospital Patient:

CBC

Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)-Pink-top (EDTA) tube is not acceptable.

Specimen Volume: Full tube

 

Collection Instructions: Immediately invert several times to mix blood.

Additional Information: Label specimen appropriately (blood for CBC).

 

Type and Screen

Specimen Type: Whole blood
Container/Tube: Pink top (EDTA)-Separator gel tube is not acceptable.

Specimen Volume: Full tube

 

Additional Information:

1. Label tube with patient’s name, date of birth, and Social Security number.

2. Specimen must be signed by person drawing the specimen. Initials are not acceptable.

3. If a type and screen is also requested or is added to this request, the specimen must have 2 signatures (phlebotomist and witness). Initials are not acceptable. Specimens without 2 signatures may be tested but will not be used for crossmatch purposes.

 

HBsAg 

Specimen Type: Serum
Container/Tube: Plain red top
Specimen Volume: Full tube

 

Additional Information: Label specimen appropriately

 

Rubella

Specimen Type: Plasma

Container/Tube:  Mint green tube

Specimen Volume:  Full tube

Additional Information:  Label specimen appropriately

 

RPR

Specimen Type: Serum
Container/Tube: Plain red top
Specimen Volume: Full tube

 

Additional Information: Label specimen appropriately (serum for RPR).

 

HIV-1/HIV-2 Ab + p24 Ag

Specimen Type:  Plasma

Container/Tube:  Pink top tube

Specimen Volume:  Full tube (7mL)

 

Day(s) Test Set Up

See individual test listings.

Reference Values

See individual test listings.

Test Classification and CPT Coding

80081-Obstetric panel with HIV-1/HIV-2 Ab + p24 Ag

86870-Antibody identification (if appropriate)

86886-Antibody titration (if appropriate)

86701--HIV-1 Multispot (if appropriate)

86702--HIV-2 Multispot (if appropriate)

87536--HIV-1 RNA  (if appropriate)

Additional Information

For BJH Laboratory Use Only

Alternate Tube:

See individual test listings.
Analyte Stability:

See individual test listings.
Laboratory Processing Instructions:

Tests performed in BJH Blood Bank and Core Lab. See individual test listings.
Note: Upon confirmation, all positive HBsAg results must be reported to the state within 7 days.

Confirmed HIV-1 positives are reported to the state within 7 days. 

Specimen Transport Temperature

Ambient