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Test Code 76480028 Enterovirus RNA Detection by PCR, CSF

Performing Laboratory

Barnes-Jewish Hospital Laboratory-Microbiology

Methodology

Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) using the Cepheid Xpert EV assay performed on the GeneXpert System

Specimen Requirements

Acceptable Specimens:

Cerebrospinal fluid (CSF): > 1 mL in a leakproof screw-capped, sterile container

Collection Procedure:
Cerebrospinal fluid (CSF)

1. Aseptically collect ≥1 mL of CSF.
2. Place in a leakproof screw-capped, sterile container.
3. Maintain sterility and forward promptly.
4. If transport is delayed, refrigerate specimen.
5. If transport is delayed greater than 72 hours, freeze specimen.
Note: 1. Specimen source is required.
           2. Laboratory medicine resident review and approval is required for CSF specimen requests.

 

Day(s) Test Set UP

Monday through Sunday
Turnaround Time:
STAT: not available
Routine:
5 hours May – October

8 hours November - April
 

Reference Values

Negative

Test Classification and CPT Coding

Test Classification:
This test is performed using the Cepheid GeneXpert Dx System EV assay. This test is U.S. Food and Drug Administration (FDA)-approved for testing of raw CSF. The performance characteristics have been determined by Barnes-Jewish Hospital Microbiology Laboratory.
CPT Code:
87498
 

Specimen Transport Temperature

CSF – Ambient/Refrigerate/Frozen/OK
If transport is delayed, refrigerate specimen.
If transport is delayed greater than 72 hours, freeze specimen.

Additional Information

For BJH Laboratory Use Only
Minimum Volume:
Cerebrospinal (CSF): 0.4 mL

Limitations

Results from the Xpert EV assay should be interpreted in conjunction with other laboratory and clinical data available to the clinician. An Xpert EV assay positive result does not rule out the presence of another pathogen like bacteria in CSF. As with any molecular assay, false positive results are always a possibility. Rare occurrences of simultaneous mixed bacterial-viral meningitis have been reported.
The Xpert EV assay is for the detection of enterovirus only. Negative test results do not rule out the presence of enterovirus. This test does not rule out the possibility of Herpes-induced meningitis or fungal meningitis; additional testing is required to rule out these infections