Sign in →

Test Code 8286 5-Hydroxyindoleacetic Acid (5-HIAA), 24 Hour, Urine

Additional Codes

Mayo Test ID

Reporting Name

5-Hydroxyindoleacetic Acid, U

Useful For

Biochemical diagnosis and monitoring of intestinal carcinoid syndrome

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type


Necessary Information

24-hour volume is required.

Specimen Required

Patient Preparation:

1. Some prescription medications could interfere with test results. The ordering provider should decide if any medications should be stopped and when they should be restarted.

2. For 48 hours before patients start their urine collection and during the 24 hours they collect urine:

Limit the following to 1 serving per day:




-Caffeinated beverages or foods


If clinically feasible, discontinue the following medications at least 48 hours before specimen collection:

-Acetaminophen (Tylenol or generic versions)



-Cough syrups

-Cold and flu medications

Supplies: Urine Tubes, 10 mL (T068)

Container/Tube: Plastic, 10-mL urine tube (T068)

Specimen Volume: 5 mL

Collection Instructions:

1. Collect a 24-hour urine specimen.

2. Add 25 mL of 50% acetic acid as preservative at start of collection. Use 15 mL of 50% acetic acid for children <5 years old.

Additional Information: See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens in Special Instructions for multiple collections.

Barnes-Jewish Hospital Note:
Submit an entire 24-hour urine collection in a white-labeled urine container.

Additional Information:

1. Starting and ending times of collection are required.
2. Follow instructions for Urine Collection in Special Instructions.

3. Urine timed measurement is performed at an additional charge per 24-hour collection (CPT Code-81050).

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Urine Refrigerated (preferred) 56 days
  Frozen  365 days

Reference Values

≤8 mg/24 hours

Day(s) and Time(s) Performed

Monday through Thursday; 11 a.m.; Friday: 2 p.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
HIAA 5-Hydroxyindoleacetic Acid, U In Process


Result ID Test Result Name Result LOINC Value
9248 5-Hydroxyindoleacetic Acid, U 1695-6
TM35 Collection Duration 13362-9
VL33 Urine Volume 3167-4

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Urine Preservative Collection Options







6N HCl

Yes (30 mL)

50% Acetic Acid

Preferred (25 mL)


Yes (5 g)


Yes (30 mL)


Yes (15 mL)

Boric Acid

Yes (10 g)

Thymol (10% in isopropanol)

Yes (10 mL)



If not ordering electronically, complete, print, and send an Oncology Test Request Form (T729) with the specimen (

Barnes-Jewish Hospital Additional Information:
For BJH Laboratory Use Only
Laboratory Processing Instructions:
BJH Chemistry will forward to the performing laboratory.