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Test Code 9933 UGT1A1-28 Genotyping for Irinotecan Toxicity

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Invader UGT1A1 molecular assay utilizes sequence-specific probes, a structure-specific cleavage enzyme, and a universal fluorescent resonance energy transfer (FRET) system

Literature Reference:

Package insert: Invader UGT1A1 molecular assay, part number 15-2655. Third Wave Technologies, Inc, 8/2005.

Specimen Requirements

Call Barnes-Jewish Hospital Molecular Diagnostics Laboratory at 314-454-8685 for collection instructions.

 

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: Full tube

Specimen Minimum Volume: 1-2 mL

Collection Instructions: Invert several times to mix blood. Clotted blood is not acceptable.

Forms: Molecular Diagnostics Laboratory Request for DNA Studies-Oncology in Special Instructions.

Day(s) Test Set Up

Friday

Sample must be received in the laboratory by Thursday at 1600
Turnaround Time:
STAT: Director approval required
Routine: 2 to 7 business days

Reference Values

An interpretive report will be provided.

Literature Reference:

Innocenti F, Undevia SD, Lyer L, et al. Genetic variants in the UDP-glucuronasyltransferase 1A1 gene predict the risk of severe neutropenia of irinotecan. J Clin Oncol 2004;22(8):1382-88.

Test Classification and CPT Coding

81350 

Additional Information

For BJH Laboratory Use Only

Analyte Stability:

Blood is stable 0 to 4 hours at ambient temperature, up to 96 hours refrigerated.

Laboratory Processing Instructions:

Test performed in BJH-Molecular Diagnostics.

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen NO