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Test Code 9935 Lactate/Pyruvate Panel, Blood

Performing Laboratory

St. Louis Children's Hospital

Last Reviewed

January 2016

Additional Information

For SLCH Laboratory use only

 

Lab Processing instructions: Let PCA tube set in wet ice for at least 10 minutes. Centrifuge specimen to separate and aliquot into a conical tube. Refrigerate specimen if received after cut-off time.
 
Processed Volume: Remove all of the supernatant without getting any of the cellular components.
A minimum of 0.3 mL supernatant should be removed and placed in conical tube.
 
Analyte Stability:

  LACTATE PYRUVATE
Ambient: NA NA
Refrigerated 8 days (supernatant) 2 days (supernatant)
Frozen NA DO NOT freeze

 

NOTES: Specimen may also be collected with 0.5 mL of whole blood but MUST be added to and mixed in an adjusted PCA tube with only 1.0 mL of perchloric acid.
Reject due to gross hemolysis.
Reject if specimen to PCA ratio is not correct.

Interpretive

Reference Range:

Lactate:
  0.5-1.5 mmol/L
Pyruvate:
  0.03-0.08 mmol/L
 
Critical Values:

Lactate:
Critical value (automatic callback): >8.0 mmol/L
Critical test results will be called initially and every 24 hours thereafter.
 
Limitations: NA
Methodology: Enzymatic

Specimen Requirements

Specimen Type: Blood


Container/Tube  
Preferred:
Perchloric acid (PCA) Vacutainer.
 
Acceptable: NA
 
Collection Volume: 1.0 mL
NOTE: minimum of 0.5 mL whole blood may be used but MUST call lab to an adjusted PCA tube for reduced volume.
 
Patient Preparation:

Remove tourniquet from patient's arm before collecting specimen.
Have ice at bedside to place specimen in after collection.
 
Sample Rejection:

Specimens collected in other than PCA tubes.
Specimens with incorrect volume of blood to PCA ratio.
Specimens that are grossly hemolyzed.
Mislabeled or unlabeled specimens.
 
Special Instructions:

1. Call lab at 314-454-4268 to have PCA tube sent for collection.
2. Collect exactly 1.0 mL of whole blood in a syringe
3. Remove needle from syringe and remove top of PCA tube. Gently push blood from syringe into PCA tube. Place top back on PCA tube and mix tube vigorously to fully deproteinize the sample.
4. Immediately place specimen in wet ice at bedside.
5. Forward to lab promptly (removing specimen from ice to send thru tube system).

Barnes-Jewish Hospital Note:
Call Barnes-Jewish Hospital Laboratory Customer Service at 314-362-1470 to obtain a perchloric acid (PCA) tube and to schedule a STAT pick-up.

Logistics

Day(s) Test Set Up: Monday-Sunday
Cut-off time: 13:00
Turnaround Time: Same day if received before cut-off time; next day if received after 1:00 p.m.
Lab Testing Section: Chemistry

General Information

KIDDOS order name: Lactate Pyruvate Panel Blood
Cerner order name: Lact/Pyruv Panel
CPT Codes:

83605: Lactate
84210: Pyruvate
LOINC Value

Lactate: 2524-7
Pyruvate: 14121-8
Synonyms:

Lab Dept

Core Lab

 

Note: Please verify that order is not for Lactate Rapid Whole Blood, for which results are available STAT.

Barnes-Jewish Hospital Additional Information:
For BJH Laboratory Use Only 
Laboratory Processing Instructions:
BJH Chemistry will forward to the performing laboratory. Send immediately on wet ice to St. Louis Children’s Hospital Laboratory. Ordered specimen with label should be forwarded directly to SLCH Chemistry.