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Test Code M201 BK Virus (BKV) DNA Detection by PCR, Quantitative  

Performing Laboratory

Barnes-Jewish Hospital Laboratory-Microbiology


Polymerase Chain Reaction (PCR)

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Specimen Requirements

Acceptable Specimens:

Urine: ≥2 mL in a leakproof screw-capped, sterile container

Whole blood: 1 lavender-top (EDTA) tube


Collection Procedure:

1. Aseptically collect ≥2 mL of urine in a leakproof screw-capped, sterile container. (Urine collected in BD VACUTAINER grey-top C & S Preservative Tube or other tube containing additives is not acceptable.)
2. Maintain sterility and forward promptly.

3. If transport is delayed, refrigerate specimen. 
Note: Specimen source is required.


Whole Blood
1. Draw blood into tube. 
2. Maintain sterility and forward promptly at ambient temperature only.
Note: 1. Specimen source is required.

2. EDTA plasma is acceptable as long as it has been stored at 2o C to  8o C.

Day(s) Test Set Up


Cut-off time: 1900 the evening prior to testing

Turnaround Time:
STAT: not available
Routine: final report available day of testing

Reference Values


Test Classification and CPT Coding

Test Classification:

Test performed using analyte-specific reagent (ASR). This test was developed and its performance characteristics determined by the St. Louis Children’s Hospital Clinical Laboratory. It has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code:




Additional Information

For BJH Laboratory Use Only

Minimum Volume:

1 mL of urine or whole blood

Alternate Tube(s)

EDTA plasma is acceptable as long as it has been stored at 2° C to 8° C.

Laboratory Processing Instructions:
BJH Microbiology will forward to the performing laboratory.

Specimen Transport Temperature

Ambient/Refrigerate OK