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Test Code M206 Ehrlichia species DNA Detection by PCR

Performing Laboratory

Barnes-Jewish Hospital Laboratory-Microbiology

Methodology

Polymerase Chain Reaction (PCR)
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Specimen Requirements

Acceptable Specimens:

Blood: 1 lavender-top (EDTA) tube

Cerebrospinal fluid (CSF): ≥1 mL in a leakproof screw-capped, sterile container

NOTE:  CSF Specimens are only tested on patients with a previously positive Blood specimen

 

Collection Procedure:

Blood

1. Draw blood into tube. Avoid hemolysis.

2. Maintain sterility and forward promptly at ambient temperature only. Specimen cannot be frozen.

Note: 1. Specimen source is required.

2. Whole blood is required for testing. (Plasma or serum is not acceptable.)

 

CSF

Note: This test can only be performed on CSF when blood has previously tested positive.

1. Aseptically collect ≥1 mL of CSF.

2. Place in a leakproof screw-capped, sterile container.

3. Maintain sterility and forward promptly.

4. If transport is delayed, refrigerate specimen. 

Note: Specimen source is required.

Day(s) Test Set Up

Monday through Saturday during active season (May-September)

Tuesday, Thursday, Saturday during non-active season

Cut-off time: 2100

Turnaround Time:

STAT: not available

Routine: final report available the day of testing

Reference Values

Negative

Test Classification and CPT Coding

Test Classification:

Test performed using analyte-specific reagent (ASR). This test was developed and its performance characteristics determined by the St. Louis Children’s Hospital Clinical Laboratory. It has not been cleared or approved by the U.S. Food and Drug Administration.

 

CPT Code:

87798

Additional Information

For BJH Laboratory Use Only

Minimum Volume:

Blood: 1 mL of EDTA whole blood
CSF: 1 mL

Laboratory Processing Instructions:
BJH Microbiology will forward to the performing laboratory.

Specimen Transport Temperature

Blood-Ambient/Refrigerate NO/Frozen NO

CSF-Ambient/Refrigerate OK