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Test Code M217 Parvovirus B19 DNA Detection by Polymerase Chain Reaction (PCR)

Performing Laboratory

Barnes-Jewish Hospital Laboratory-Microbiology

Methodology

Polymerase Chain Reaction (PCR)
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Specimen Requirements

Acceptable Specimens:

 

Bone Marrow: 1 lavender-top (EDTA) tube

 

Fluids [amniotic, cerebrospinal (CSF), pericardial, peritoneal dialysis (PDF), peritoneal, pleural, synovial]: ≥1 mL in a leakproof screw-capped, sterile container.

 

Plasma or whole blood: 1 lavender-top (EDTA) tube

 

 

Collection Procedure:

Bone Marrow

 1. Completely fill tube. Avoid hemolysis.
2. Maintain sterility and forward promptly at ambient temperature only. Specimen cannot be frozen.
Note: Specimen source is required

 

Fluids [amniotic, cerebrospinal (CSF), pericardial, peritoneal dialysis (PDF), peritoneal, pleural, synovial]

1. Aseptically collect ≥1 mL of fluid.
2. Place in a leakproof screw-capped, sterile container.
3. Maintain sterility and forward promptly

4. If transport is delayed, refrigerate specimen. 
Note: Specimen source is required

 

Plasma or Whole Blood

1. Draw blood into tube. Avoid hemolysis.
2. Maintain sterility and forward promptly at ambient temperature only. Specimen cannot be frozen.
Note:
Specimen source is required

Day(s) Test Set Up

Monday,Tuesday, Wednesday, Thursday, Friday, Sunday

 

Turnaround Time:
STAT: not available
Routine: final report available the day of testing

Reference Values

Negative

Test Classification and CPT Coding

Test Classification:

Test performed using analyte-specific reagent (ASR). This test was developed and its performance characteristics determined by the St. Louis Children’s Hospital Clinical Laboratory. It has not been cleared or approved by the U.S. Food and Drug Administration.

 

CPT Code:

56075-Blood

83151-All other approved specimen types

Additional Information

For BJH Laboratory Use Only

 

Minimum Volume:

Bone Marrow: 0.4 mL from a lavender-top (EDTA) tube

Fluids [amniotic, cerebrospinal (CSF), pericardial, peritoneal dialysis (PDF), peritoneal, pleural, synovial]: 0.4 mL

Plasma or whole blood: 1 mL

 

Laboratory Processing Instructions:

BJH Microbiology will forward to the performing laboratory.

 

 

Specimen Transport Temperature

Plasma or Whole Blood, Bone Marrow-Ambient/Refrigerate NO/Frozen NO

 

Amniotic Fluid, CSF, Pericardial Fluid, Peritoneal Dialysis Fluid (PDF), Peritoneal Fluid, Pleural Fluid, Synovial Fluid -Ambient/Refrigerate OK