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Test Code M221 Varicella-Zoster Virus (VZV) DNA Detection by PCR

Performing Laboratory

Barnes-Jewish Hospital Laboratory-Microbiology

Methodology

Polymerase Chain Reaction (PCR)
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Specimen Requirements

Acceptable Specimens:

Blood: 1 lavender-top (EDTA) tube
Cerebrospinal fluid (CSF), synovial fluid: ≥1 mL in a leakproof screw-capped, sterile container
Ocular fluid: ≥0.5 mL in a leakproof screw-capped, sterile container
 

Collection Procedure:

 

Blood

Call Laboratory Medicine resident at digital pager 314-747-1320 for approval before obtaining the specimen.
1. Draw blood into tube. Avoid hemolysis.

2. Maintain sterility and forward promptly at ambient temperature only. Specimen cannot be frozen.

Note: 1. Specimen source is required.

2. Whole blood is required for testing. (Plasma or serum is not acceptable.)

 

CSF, Synovial Fluid
1. Aseptically collect ≥1 mL of CSF.
2. Place in a leakproof screw-capped, sterile container.
3. Maintain sterility and forward promptly.

4. If transport is delayed, refrigerate specimen. 
Note: 1. Specimen source is required.

           2. Laboratory medicine resident review and approval is required for CSF specimen requests.

 

 

Ocular Fluid
1. Aseptically collect ≥0.5 mL of fluid.
2. Place in a leakproof screw-capped, sterile container.
3. Maintain sterility and forward promptly.

4. If transport is delayed, refrigerate specimen. 
Note: Specimen source is required.

Day(s) Test Set Up

Tuesday, Wednesday, Friday 

Cut-off time: 2100 the evening prior to testing

Turnaround Time:
STAT: not available
Routine: final report available the day of testing

Reference Values

Negative

Test Classification and CPT Coding

Test Classification:

Test performed using analyte-specific reagent (ASR). This test was developed and its performance characteristics determined by the St. Louis Children’s Hospital Clinical Laboratory. It has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code:

87798

Additional Information

For BJH Laboratory Use Only

Minimum Volume:

Blood: 1 mL of EDTA whole blood
CSF, Synovial Fluid: 1 mL
Ocular fluid: any volume
Laboratory Processing Instructions:
BJH Microbiology will forward to the performing laboratory.

Specimen Requirements Additional Information

Blood-Ambient/Refrigerate NO/Frozen NO

CSF, Synovial Fluid-Ambient/Refrigerate OK-Frozen NO

Ocular Fluid-Ambient/Refrigerate OK