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Test Code M228 Bartonella henselae DNA Detection by PCR

Performing Laboratory

Barnes-Jewish Hospital Laboratory-Microbiology

Methodology

Polymerase Chain Reaction (PCR)
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

Specimen Requirements

NOTE: This test detects Bartonella henselae only (Cat Scratch Disease)
Bone Marrow: 1 lavender-top (EDTA) tube
Lymph node: ≥1 g in a leakproof screw-capped, sterile container
Lesion:  1 swab in viral transport medium
Tissue: ≥1 g in a leakproof screw-capped, sterile container


 

Collection Procedure:

Bone Marrow

Call Laboratory Medicine resident at 314-747-1320 for approval before obtaining the specimen.
1. Completely fill tube. Avoid hemolysis.
2. Maintain sterility and forward promptly at ambient temperature only. Specimen cannot be frozen.
Note: Specimen source is required.

 

Lesion
Call Laboratory Medicine resident at 314-747-1320 for approval before obtaining the specimen.
1. Obtain Viral Transport Medium Collection Kit.
2. Collect specimen using plastic shaft Dacron swab.
3. Place swab in vial, break or cut off shaft of swab, discard shaft, tightly cap tube.
4. Maintain sterility and forward promptly.
5. If transport is delayed, refrigerate specimen.
Note: Specimen source is required.
 
Lymph Node

1. Aseptically collect ≥1 g of tissue.
2. Place specimen in a leakproof screw-capped, sterile container.
3. Maintain sterility and forward promptly.
4. If transport is delayed, refrigerate specimen.
Note: Specimen source is required.

 

Tissue
Call Laboratory Medicine resident at 314-747-1320 for approval before obtaining the specimen.
1. Aseptically collect ≥1 g of tissue.
2. Place specimen in a leakproof screw-capped, sterile container.
3. Maintain sterility and forward promptly.
4. If transport is delayed, refrigerate specimen.

 

Day(s) Test Set Up

Tuesday, Thursday, Sunday

Cut-off time: 2100

Turnaround Time:
STAT: not available
Routine: final report available the day of testing

Reference Values

Negative

Test Classification and CPT Coding

Test Classification:

Test performed using analyte-specific reagent (ASR). This test was developed and its performance characteristics determined by the St. Louis Children’s Hospital Clinical Laboratory. It has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code:

87798

Additional Information


For BJH Laboratory Use Only

Minimum Volume:
Bone Marrow: 1 lavender-top (EDTA) tube
Lesion:  1 swab in viral transport medium
Lymph node: 1 g
Tissue: 1 g

Laboratory Processing Instructions:
BJH Microbiology will forward to the performing laboratory.

Specimen Transport Temperature

Bone Marrow-Ambient/Refrigerate NO/Frozen NO
Lesion, Lymph node, Tissue - Ambient/Refrigerate OK