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Test Code M231 Norovirus RNA Detection by PCR

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Polymerase Chain Reaction (PCR)
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)                                                

Specimen Requirements

Acceptable Specimens
≥1 mL of fresh liquid or non-formed stool  in a leakproof screw-capped, sterile container

 

Unacceptable Specimen:
Solid or formed stool
 
Collection Procedure:
Call Laboratory Medicine resident at digital pager 314-747-1320 for approval before obtaining the specimen.

1. Collect ≥1 mL of fresh liquid or non-formed stool.
2. Place specimen in a leakproof screw-capped, sterile container. (Stool in container with preservative is not acceptable.)
3. Maintain sterility and forward promptly.
4. If transport is delayed, refrigerate specimen. If specimen will be in transport > 4 hours, transport with ice pack.
Note: 1. Specimen source is required.
2. Specimen should not be collected following a barium enema.
3. Previously frozen specimen is acceptable.                                                

Day(s) Test Set Up

Monday through Sunday
Cut-off time: 2100 the evening prior to testing                                      
Turnaround Time:
STAT: not available
Routine: final report available the day of testing

Reference Values

Norovirus RNA not detected (Negative)

Test Classification and CPT Coding

87798

Additional Information

For BJH Laboratory Use Only
Minimum Volume
1 mL of fresh liquid or non-formed stool  in a leakproof screw-capped, sterile container

Laboratory Processing Instructions:
BJH Microbiology will forward to the performing laboratory

Specimen Transport Temperature

Ambient OK (within 4 hours)/Refrigerate OK/Frozen OK