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Test Code M232 Respiratory Pathogen Multiplex PCR

Performing Laboratory

  Barnes-Jewish Hospital Molecular Infectious Disease Laboratory                          

Methodology

Polymerase chain reaction (PCR) with melt curve analysis

 

This test is capable of detecting the following respiratory pathogens:
Bordetella pertussis, Bordetella parapertussis, Chlamydophila pneumoniae, Mycoplasma pneumoniae, Adenovirus, Coronavirus HKU1, Coronavirus NL63, Coronavirus 229E, Coronavirus OC43, Influenza A, Influenza A subtype H1, Influenza A subtype H3, Influenza A subtype 2009 H1, Influenza B, Metapneumovirus, Parainfluenza 1, Parainfluenza 2, Parainfluenza 3, Parainfluenza 4, RSV and Rhinovirus/Enterovirus

                           

Specimen Requirements

Acceptable Specimens

Nasopharyngeal (NP): 1 swab in viral transport medium
Respiratory (bronchial alveolar lavage, bronchial washing, sputum, tracheal aspirate): ≥1 mL in a leakproof screw-capped, sterile container

 

Collection Procedure

Nasopharyngeal 
1. Call Barnes-Jewish Hospital Laboratory Customer Service at 314-362-1470 to obtain Viral Transport Medium Collection Kit.

2. Collect specimen using plastic shaft swab(s) provided.
3. Place swab(s) in vial, break or cut off shaft of swab(s), discard shaft, and tightly cap vial.
4. Maintain sterility and forward promptly.

5. If transport is delayed, refrigerate specimen
Note: Specimen source is required.

 

Respiratory (Bronchial Alveolar Lavage, Bronchial Washing, Sputum, Tracheal Aspirate) 
1. Aseptically collect ≥1 mL of specimen.
2. Place in a leakproof screw-capped, sterile container.
3. Maintain sterility and forward promptly.

4. If transport is delayed, refrigerate specimen. 
Note: Specimen source is required.  

 

 

Day(s) Test Set Up

 Monday through Sunday

 

Turnaround Time (STAT, Routine)    

STAT: not available

Routine: 3-12 hours                                  

 

 

Reference Values

Respiratory Pathogen  Nucleic Acids Not Detected (Negative)         

Test Classification and CPT Coding

CPT Code:               

87633                                        

Additional Information

For BJH Laboratory Use Only

 

Minimum Volume: 
Nasopharyngeal (NP): 1 swab in viral transport medium

Respiratory (bronchial alveolar lavage, bronchial washing, sputum, tracheal aspirate): 1 mL in a leakproof screw-capped, sterile container

 

Laboratory Processing Instructions    

    BJH Microbiology will forward to the performing laboratory.  

 

 

Specimen Transport Temperature

        

Nasopharyngeal Swab, Respiratory-Ambient/Refrigerate OK