Sign in →

Test Code M301 KOH Preparation

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Specimen Requirements

Acceptable Specimens:
Abscess, aspirate, or wound: ≥1 mL in a leakproof screw-capped, sterile container
Biopsy or tissue: ≥1 g in a leakproof screw-capped, sterile container
Eye: conjunctiva and cornea-1 BD ESwab Minitip Collection Kit; ocular fluid (anterior chamber, aqueous humor, vitreous)-≥0.5 mL in a leakproof screw-capped, sterile container
Note: Only 1 swab is required when this test and #M012 Culture, Fungus and Yeast are ordered together. Swabs are not optimal for KOH testing.  If #M023 Culture, Mycobacterium, Other than Blood or Bone Marrow is also requested, collect an additional specimen using a second BD ESwab Minitip Collection Kit.
Fluid (Joint, Pericardial, Synovial, etc.): ≥1 mL in a leakproof screw-capped, sterile container
 
Unacceptable Specimens:
Blood, cerebrospinal fluid (CSF), drainage, environmental, respiratory (bronchial brush, bronchial washing, bronchial alveolar lavage, tracheal aspirate), oral, peritoneal dialysis fluid, specimen received on a culture swab, stool, throat, or urine

 

Collection Procedure:

Abscess, Aspirate, or Wound
1. Disinfect surface of abscess or wound.
2. Aseptically collect purulent material by aspiration (≥1 mL). Frequently, viable organisms are only associated with abscess wall, so adequately sample this area. If material is aspirated, aseptically inject specimen into a leakproof screw-capped, sterile container or a plain, red-top tube.
3. Maintain sterility and forward promptly.

4. If transport is delayed, refrigerate specimen.
Note: Specimen source is required.

 

Biopsy or Tissue
1. Aseptically collect ≥1 g of tissue from border of lesion. Because there may be only a few viable organisms in a lesion, specimen must be large enough to permit their recovery.
2. Place specimen in a leakproof screw-capped, sterile container.
3. Maintain sterility and forward promptly.

4. If transport is delayed, refrigerate specimen.
Note: Specimen source is required.

 

Eye

1. Conjunctiva or cornea
Call Barnes-Jewish Hospital Laboratory Customer Service at 314-362-1470 to obtain BD ESwab Minitip Collection Kit.
A. Collect specimen before application of topical anesthetics because topical anesthetics possess antimicrobial activity.
B. Cleanse skin around eye with mild antiseptic.
C. Gently remove makeup and ointment with sterile cotton and saline.
D. Collect specimen by passing  swab 2 times over lower conjunctiva or over cornea. Avoid eyelid border and lashes (culture these separately in a similar fashion, if indicated).
E. Place swab in transport tube following instructions on kit. Specimen is unacceptable if received without fluid or swab in the transport tube.
F. Maintain sterility and forward promptly.

G. If transport is delayed, refrigerate specimen.
 

2. Ocular fluid (anterior chamber, aqueous humor, vitreous)
A. Aseptically collect ≥0.5 mL of fluid.
B. Place specimen in a leakproof screw-capped, sterile container.
C. Maintain sterility and forward promptly.

D. If transport is delayed, refrigerate specimen.
Note: Specimen source is required.

 

Fluid (Joint, Pericardial, Synovial, etc.)
1. Aseptically collect ≥1 mL of fluid.
2. Place specimen in a leakproof screw-capped, sterile container.
3. Maintain sterility and forward promptly.

4. If transport is delayed, refrigerate specimen.
Note: Specimen source is required.

Day(s) Test Set Up

Monday through Sunday

Cut-off time: 1430

Turnaround Time:
STAT: not available
Routine: results available the day of testing

Reference Values

No fungal elements seen

Literature Reference:

Larone, Davise H. Medically important fungi-a guide to identification. 5th ed. Washington, DC: American Society for Microbiology; 2011;344.

Test Classification and CPT Coding

87210

Additional Information

For BJH Laboratory Use Only
Minimum Volume: 
Abscess, aspirate, or wound: 0.5 mL in a leakproof screw-capped, sterile container
Biopsy or tissue: 1 g in a leakproof screw-capped, sterile container
Eye: conjunctiva or cornea-1 BD ESwab Minitip Collection Kit; ocular fluid (anterior chamber, aqueous humor, vitreous)-0.3 mL in a leakproof screw-capped, sterile container
Fluids (joint, pericardial, synovial, etc.): 0.5 mL in a leakproof screw-capped, sterile container
Laboratory Processing Instructions:
Test performed in BJH Microbiology.

Specimen Transport Temperature

Ambient/Refrigerate OK