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Test Code PTHFN Parathyroid Hormone, Fine-Needle Aspiration Biopsy (FNAB)-Needle Wash

Reporting Name

PTH, FNAB, Needle Wash

Useful For

An adjunct to cytology examination of fine-needle aspiration specimens to confirm or exclude presence of parathyroid tissue in the biopsied area

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Fine Needle Wash

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Fine Needle Wash Frozen (preferred) 30 days
  Refrigerated  4 hours

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Monday through Friday; 6 a.m.-9 p.m., Saturday; 6:30 a.m.-1 p.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

83970

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PTHFN PTH, FNAB, Needle Wash In Process

 

Result ID Test Result Name Result LOINC Value
PTHF PTH, FNAB, Needle Wash No LOINC Needed
SITEA Site 39111-0


Shipping Instructions


Send specimen frozen to Mayo Medical Laboratories for analysis.



Necessary Information


The biopsied site of each specimen must be clearly identified in LIS and/or batch sheet.



Specimen Required


Patient Preparation: Twelve hours before this test, do not take multivitamins or dietary supplements containing biotin or vitamin B7 that are commonly found in hair, skin and nail supplements and multivitamins.

Collection Container/Tube: Plain, plastic, screw-top tube

Specimen Volume: 1 to 1.5 mL

Collection Instructions:

1. Needle wash specimens for analysis should be collected in conjunction with cytology specimens.

2. Have saline available prior to start of procedure. Saline is the only acceptable solution for needle washings.

3. After each fine-needle aspiration biopsy (FNAB) has been collected and the material in the needle has been expelled onto a slide for cytologic analysis, attach the used FNAB needle to an empty syringe.

4. Withdraw between 0.10 mL and 0.25 mL of saline up through the needle until the saline starts to fill the hub of the needle or end of the syringe.

5. Expel this fluid back through the needle into a separate plastic aliquot tube. This is the needle washing used for analysis.

6. Repeat steps 2 through 4 for each needle pass of the same biopsied site and empty into the same tube, accumulating a total of 0.5 mL to 1.5 mL of fluid to send to the laboratory. (If more than 1 site is biopsied, see Additional Information)

7. Inspect specimen for visible blood or tissue contamination:

 -a. If bloody, centrifuge specimen and transfer supernatant to a new plastic aliquot tube (5-mL standard tube) to send to laboratory. The supernatant, not the cellular material, is used for analysis.

 -b. If specimen is clear, centrifugation is not necessary.

8. Refrigerate within 1 to 2 hours of collection and freeze within 2 to 4 hours of collection.

Additional Information:

1. If more than 1 site is biopsied, each washing material should be submitted on a separate tube and under a different order number.

2. A minimum of 0.5 mL is required for testing; however, the total collection volume should not exceed 1.5 mL. Sample volumes outside these parameters may be rejected.

3. Do not send saline control. This test has been validated to rule-out saline matrix effect.


Method Name

Electrochemiluminescence Immunoassay

Special Instructions