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Test Code 0227 Uric Acid, Urine, Timed

Performing Laboratory

Barnes-Jewish Hospital Laboratory


Enzymatic (Uricase)

Literature Reference:

Package insert: Uric acid UA2. Roche Corp., 10/2015.

Specimen Requirements

Specimen Type: Urine, timed

Container/Tube: Plain, brown urine container-Specimen collected in a container with an acid or base is not acceptable.

Specimen Volume: Entire collection


Collection Instructions: No additive. Refrigerate specimen during collection.

Additional Information:

1. Starting and ending times of collection are required.

2. Follow instructions in Urine Collection in Special Instructions.

Day(s) Test Set Up

Monday through Sunday
Turnaround Time:
STAT: 1 hour after receipt in laboratory
Routine: 2 to 4 hours after receipt in laboratory

Reference Values

No established reference intervals

Literature Reference:

Tietz, Burtis CA, Ashwood ER, Bruns DE eds: Textbook of Clinical Chemistry and Molecular Diagnostics 5th ed: Chapter 25, p.669, Elsevier, St. Louis, MO 2012.

Test Classification and CPT Coding

81050-Volume measurement for timed collection

84560-Uric acid

Additional Information

For BJH Laboratory Use Only

Analyte Stability:

See Analyte Stability Chart in Special Instructions.

Specimen must be kept refrigerated during collection and before delivery to limit bacterial growth.

Laboratory Processing Instructions:

Test performed in BJH Core Lab. Mix urine well. Record volume, period, and pH on request form. Using 13x100 test tubes, prepare a 7-mL aliquot. Check the pH. If pH is <9, add 6 N NaOH in 1 drop increments until the pH is between 9 and 10. Do not over adjust. Heat for 10 minutes at 56° C. Deliver to the automated line for centrifugation and delivery to Cobas 8000 for analysis. Completed specimen is stored refrigerated for 5 days.

Specimen Transport Temperature