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Test Code 09991 pH, Pleural Fluid

Performing Laboratory

Barnes-Jewish Hospital Laboratory


Ion-Specific Electrode (ISE) (pH Meter)

Literature References:

1. Reference manual: ABL 800 Series.  Radiometer America, Bronshoj Denmark, 2009.

2. pH Meter: The pH 11 and pH 110 hand-held pH/mV/Temperature/RS 232 meter instruction manual. Oakton Instruments; Vernon Hill, IL 60061.

Specimen Requirements

Specimen must arrive within 1 hour of collection.


Specimen Type: pleural Fluid

Container/Tube: Clean container or syringe

Specimen Volume: 5 mL


Collection Instructions:

1. Obtain specimen and place into a clean container or syringe.

2. If collected in a clean container, immediately draw 5 mL of fluid into a syringe.

3. Expel any air and cap with a Luer-Lok.

4. Place specimen in ice and send immediately to the lab.

Day(s) Test Set Up

Monday through Sunday
Turnaround Time:
STAT: 1 hour after receipt in laboratory
Routine: not available

Reference Values

Pleural fluid pH is normally approximately 7.64. pH <7.2 is indicative of complicated parapneumonic effusions.


Literature References:

Block, Darci R. Tietz Textbook of clinical Chemistry and Molecular Diagnositics. Elsevier Sixth Edition 2017. Body Fluids Abstract p. 925

Yataco, Jose C, Cleveland Clinic Journal of Medicine. Pleural effusions: Evaluation and Management. October 2005. Volume 72, Number 10.


Test Classification and CPT Coding


Additional Information

For BJH Laboratory Use Only

Analyte Stability:

Not established; performed upon receipt

Laboratory Processing Instructions:

Test performed in BJH Core lab. Specimen must be received in a syringe and on ice within 1 hour of collection. Deliver to the blood gas workstation immediately.

Specimen Transport Temperature

On wet ice

LOINC Code Information

Name LOINC Code
pH, Fluid 2748-2