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Test Code 1335 Methadone, Urine

Performing Laboratory

Barnes-Jewish Hospital Laboratory


Screen:  Kinetic Interaction of Microparticles in Solution (KIMS) 

Confirmation:  Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Testing Algorithm:

If the specimen is positive by the screening method, confirmation will be performed automatically by an alternate method, at an additional charge.

Literature Reference:

Methadone II (MDN2) package insert, Roche Diagnostics.

Specimen Requirements

Specimen Type: Urine, random

Container/Tube: Yellow-top urine collection tube or plain red-top tube (full)

Specimen Volume: 10 mL

Collection Instructions: No additive.

Additional Information: See Urine Collection in Special Instructions for additional information.

Day(s) Test Set Up

Monday through Sunday
Turnaround Time:
STAT: Screening result available 1 hour after receipt in laboratory. Confirmation of presumptive positive results within 4 hours.
Routine: Screening result available 3 hours after receipt in laboratory. Confirmation of presumptive positive results within 4 hours.

Reference Values

None detected

Immunoassay screen cut-off level: 300 ng/mL

Note: Drug results are to be used only for medical purposes. All results, especially unconfirmed screening results, must not be used for non medical purposes.

Test Classification and CPT Coding

80307-Drug screen

80358-Drug confirmation, (if appropriate) (G0480)

Additional Information

For BJH Laboratory Use Only

Analyte Stability:
Specimens may be stored at ambient temperature for up to 1 week. Freeze specimens after 1 week.
Laboratory Processing Instructions:

Test performed in BJH Core Lab.  Processed by automated line for delivery to the Cobas 8000. Completed specimens are stored refrigerated for 5 days. 

Specimen Transport Temperature


Special Instructions

LOINC Code Information

Name LOINC Code
Methadone, Urine 19550-3