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Test Code 1787 Digoxin, Plasma

Performing Laboratory

Barnes-Jewish Hospital Laboratory


Kinetic interaction of microparticles in solution (KIMS)

Literature References:

Package Insert: Digoxin DIG. Roche Corporation 02/2012.

Specimen Requirements

Specimen Type: Plasma
Container/Tube: Mint-green top (lithium heparin)
Specimen Volume: Full tube

Collection Instructions: Draw specimen no earlier than 60 minutes prior to dose. Absorption is complete by 8 hours, therefore, a specimen drawn 8 or more hours after the last dose is acceptable.

Additional Information: 

  1. Endogenous substances such as DLIF (Digoxin-like immunoreactive factors) may interfere with this assay by yielding slightly elevated results. DLIF are observed primarily in samples from neonates, pregnant women, and acute care patients with renal or hepatic failure.
  2. The manufacturer of Digoxin Immune Fab (Antibody fragment therapy) has stated that no immunoassay technique is suitable for quantitating Digoxin in serum from patients undergoing this treatment.


Day(s) Test Set Up

Monday through Sunday

Turnaround Time:
STAT: 1 hour after receipt in laboratory 
Routine: 2-4 hours after receipt in laboratory

Reference Values

Therapeutic concentration: 0.5-0.8 ng/mL

Digoxin concentrations as high as 2.0 ng/mL may be useful in treating atrial fibrillation.

Critical result (automatic call-back): ≥2.4 ng/mL

Literature References:


  • The Digitalis Investigation Group. The effect of digoxin on mortality and morbidity in patients with heart failure. N. Engl. J. Med. 336: 525-532 (1997).
  • Ahmed A, Pitt B, Rahimtoola SH, Waagstein F, White M, Love TE, Braunwald E. Effects of digoxin at low serum concentrations on mortality and hospitalization in heart failure: a propensity-matched study of the DIG trial. Int. J. Cardiol. 123: 138-46 (2008).
  • Goldberger ZD, Goldberger AL. Therapeutic ranges of serum digoxin concentrations in patients with heart failure. Am. J. Cardiol. 109: 1818-1821 (2012).




Test Classification and CPT Coding


Additional Information

For BJH Laboratory Use Only
Alternate Tube:

1 dark green-top (lithium or sodium heparin) tube

1 red-top tube

1 lavender-top (EDTA) tube
Separator gel tube is acceptable

Analyte Stability:

See Analyte Stability Chart in Special Instructions.

Laboratory Processing Instructions:

Test performed in BJH Core Lab.  Processed by automated line for delivery to the Cobas 8000. Completed specimens are stored refrigerated for 5 days. 

Specimen Transport Temperature


Special Instructions

LOINC Code Information

Name LOINC Code
Digoxin, Plasma 10535-3