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Test Code 2543 LDL-Cholesterol (Low-Density Lipoprotein), Direct

Performing Laboratory

Barnes-Jewish Hospital Laboratory



Note: This test is automatically performed when the triglyceride in #1169 Lipid Panel is ≥400 mg/dL.

Literature Reference:

Package insert: LDL reagent from Roche. 6/2015.

Specimen Requirements

Patient Preparation:

1. A fasting specimen (12 to 14 hour, overnight) is required if triglycerides or LDL, calculated are ordered along with this test, as triglycerides are affected by recent dietary intake. A fasting specimen is not required if triglycerides or LDL, calculated are not ordered.

2. Patients should be following ordinary eating habits and not be acutely ill, losing weight, pregnant, or had a myocardial infarction within the last 3 months.

Specimen Type: Plasma

Container/Tube: Mint-green top (lithium heparin)

Specimen Volume: Full tube


Day(s) Test Set Up

Monday through Sunday
Turnaround Time:
STAT: 1 hour after receipt in laboratory
Routine: 2 to 4 hours after receipt in laboratory

Reference Values

Optimal: <100 mg/dL
Near optimal: 100-129 mg/dL
Borderline: 130-159 mg/dL
High: >160 mg/dL

Literature Reference:

National cholesterol education program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (Adult Treatment Panel III). Circulation 2004;110:227.

Test Classification and CPT Coding


Additional Information

For BJH Laboratory Use Only
Alternate Tube:

1 dark green-top (lithium heparin) tube
1 red-top tube

Analyte Stability:

See Analyte Stability Chart in Special Instructions.

Laboratory Processing Instructions:

Test performed in BJH Core Lab.  Processed by automated line for delivery to the Cobas 8000. Completed samples are stored refrigerated for five days.

Specimen Transport Temperature


Special Instructions

LOINC Code Information

Name LOINC Code
Cholesterol in LDL 18262-6