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Test Code 9619 14-3-3 Protein, Cerebrospinal Fluid (CSF)

Performing Laboratory

National Prion Disease Pathology Surveillance Center


Note: Test searches for the presence of the 14-3-3 protein. The 14-3-3 protein is a marker for some prion diseases, such as Creutzfeldt-Jakob disease (CJD), when a number of other neurodegenerative conditions are excluded.

Specimen Requirements

Specimen Type: Cerebrospinal fluid (CSF)

Container/Tube: Screw-capped, plastic vial

See Body Fluid Collection Guidlines under Special Instructions.

Specimen Volume: 2-5 mL

Collection Instructions:

1. Obtain CSF by lumbar puncture.

2. Discard the first 2 mL of CSF that flows from the tap.

3. Collect 2 mL to 5 mL of CSF, avoiding bloody tap.

4. Forward promptly on ice.
Additional Information: Include relevant clinical information, name, phone number, mailing address, and e-mail address (if applicable) of ordering physician.

Day(s) Test Set Up

Days set up vary

Turnaround Time:
STAT: not available
Routine: 8 days after receipt at reference laboratory

Reference Values

No immunoreactivity

Test Classification and CPT Coding



87798 (if appropriate)


Additional Information

For BJH Laboratory Use Only

Laboratory Processing Instructions:
BJH Chemistry will forward to the performing laboratory. Freeze 1 mL to 2 mL CSF as quickly as possible (preferably within 20 minutes) and store at -80° C. Complete National Prion Testing request form. Ship on dry ice, Monday through Wednesday only.

Specimen Transport Temperature

On wet ice

Special Instructions