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Test Code M230 Adenovirus DNA Detection by PCR, Qualitative

Performing Laboratory

Barnes-Jewish Hospital Laboratory


Polymerase Chain Reaction (PCR) performed on the Focus Integrated Cycler

Specimen Requirements


Acceptable Specimens:

Whole blood (Lavender-top/EDTA tube), Bronchoalveolar lavage, Bronchial wash, tracheal aspirate, or Nasopharyngeal Swab in viral transport medium


Collection Procedure:


1. Draw blood into tube. Avoid hemolysis.

2. Maintain sterility and forward promptly at ambient temperature only. 


Bronchial Alveolar Lavage (BAL), Bronchial Washes, or Tracheal Aspirate

1. Aseptically collect at least 1.0 mL of BAL, bronchial wash specimen, or tracheal aspirate.

2. Place specimen in a labeled, screw-capped, sterile container. 

3. Maintain sterility and forward promptly.


1. Specimen source is required


Nasopharyngeal Swab

1. Obtain a vial of universal/viral transport medium (UTM) with collection swab

2. Collect nasopharyngeal specimen

3. Place the swab in labeled UTM vial, break off shaft of swab and discard excess piece of

shaft. To prevent leaking, make sure the swabs do not interfere with tightening cap on vial.


1. Specimen source is required





Day(s) Test Set Up



Turnaround Time:
STAT: not available
Routine: 1-3 days, Final report available the day of testing

Reference Values


Test Classification and CPT Coding

Test Classification:

This assay utilizes ASR’s (analyte specific reagents) and is not FDA cleared.  This test was developed and its performance has been evaluated by the Barnes Jewish Molecular Infectious Disease Laboratory for performance on whole blood, lower respiratory specimens, and NP swabs.

Assay Limit of Detection:

Lower Respiratory Samples:  2,000 copies/mL

Blood:  20,000 copies/mL

NP swabs: 2,000 copies/mL


-The detection of viral nucleic acid is dependent upon proper sample collection, transport, handling and storage. Failure to observe proper procedures in any one of these steps can lead to incorrect results.

-False-negative results may occur if the viruses are present at a level that is below the analytical sensitivity of the assay or if the virus has genomic mutations, insertions, deletions, or rearrangements or if performed very early in the course of illness


CPT Code:


Additional Information

For BJH Laboratory Use Only

Minimum Volume: 
Blood: 1 lavender-top (EDTA) tube

Bronchial Alveolar Lavage (BAL), Bronchial Washes, or Tracheal Aspirate: 0.5 mL


Laboratory Processing Instructions:
BJH Microbiology will forward to the performing laboratory.

Specimen Transport Temperature

Blood-Ambient/Refrigerate /Frozen OK

Bronchial Alveolar Lavage (BAL), Bronchial Washes, or Tracheal Aspirate- Ambient/Refrigerate/Frozen OK

Swabs- Ambient/Refrigerate/Frozen OK

Refrigerated specimens must be tested within 7 days
Frozen specimens must be tested within 30 days