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Test Code M232 Respiratory Pathogen Multiplex PCR

Performing Laboratory

  Barnes-Jewish Hospital Molecular Infectious Disease Laboratory                          


Polymerase chain reaction (PCR) with melt curve analysis


This test is capable of detecting the following respiratory pathogens:
Bordetella pertussis, Bordetella parapertussis, Chlamydophila pneumoniae, Mycoplasma pneumoniae, Adenovirus, Coronavirus HKU1, Coronavirus NL63, Coronavirus 229E, Coronavirus OC43, Influenza A, Influenza A subtype H1, Influenza A subtype H3, Influenza A subtype 2009 H1, Influenza B, Metapneumovirus, Parainfluenza 1, Parainfluenza 2, Parainfluenza 3, Parainfluenza 4, RSV and Rhinovirus/Enterovirus


Specimen Requirements

Acceptable Specimens

Nasopharyngeal (NP): 1 swab in viral transport medium
Respiratory (bronchial alveolar lavage, bronchial washing, sputum, tracheal aspirate): ≥1 mL in a leakproof screw-capped, sterile container


Collection Procedure

1. Call Barnes-Jewish Hospital Laboratory Customer Service at 314-362-1470 to obtain Viral Transport Medium Collection Kit.

2. Collect specimen using plastic shaft swab(s) provided.
3. Place swab(s) in vial, break or cut off shaft of swab(s), discard shaft, and tightly cap vial.
4. Maintain sterility and forward promptly.

5. If transport is delayed, refrigerate specimen
Note: Specimen source is required.


Respiratory (Bronchial Alveolar Lavage, Bronchial Washing, Sputum, Tracheal Aspirate) 
1. Aseptically collect ≥1 mL of specimen.
2. Place in a leakproof screw-capped, sterile container.
3. Maintain sterility and forward promptly.

4. If transport is delayed, refrigerate specimen. 
Note: Specimen source is required.  



Day(s) Test Set Up

 Monday through Sunday


Turnaround Time (STAT, Routine)    

STAT: not available

Routine: 3-12 hours                                  



Reference Values

Respiratory Pathogen  Nucleic Acids Not Detected (Negative)         

Test Classification and CPT Coding

CPT Code:               


Additional Information

For BJH Laboratory Use Only


Minimum Volume: 
Nasopharyngeal (NP): 1 swab in viral transport medium

Respiratory (bronchial alveolar lavage, bronchial washing, sputum, tracheal aspirate): 1 mL in a leakproof screw-capped, sterile container


Laboratory Processing Instructions    

    BJH Microbiology will forward to the performing laboratory.  



Specimen Transport Temperature


Nasopharyngeal Swab, Respiratory-Ambient/Refrigerate OK