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Methadone, Urine

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Screen:  Kinetic Interaction of Microparticles in Solution (KIMS) 

 

Testing Algorithm:

Samples containing greater than 300 ng/mL of methadone or other cross-reacting substances are reported as screen positive, presumptive.  Results are presumptive for medical purposes only.  If the screen is positive, confirmation LAB3879 will be performed automatically by LC-MS/MS, at an additional charge.  

 

Literature Reference:

Methadone II (MDN2) package insert, Roche Diagnostics.

Specimen Requirements

Specimen Type: Urine, random

Container/Tube: Yellow-top urine collection tube or plain red-top tube (full)

Specimen Volume: 10 mL

Collection Instructions: No additive.

Additional Information: See Urine Collection in Special Instructions for additional information.

Day(s) Test Set Up

Monday through Sunday
Turnaround Time:
STAT: Screening result available 1 hour after receipt in laboratory. Confirmation performed within 24 hours.
Routine: Screening result available 3 hours after receipt in laboratory. Confirmation performed within 24 hours.

Test Classification and CPT Coding

80307-Drug screen

80358-Methadone, (if appropriate) (G0480)

Reference Values

None detected

Positive screen will be confirmed by LC-MS/MS.

The following drug(s) will be confirmed for positive screen: methadone and EDDP.

Immunoassay screen cut-off level: 300 ng/mL.

Note: Drug results are to be used only for medical purposes. All results, especially unconfirmed screening results, must not be used for non medical purposes.

Specimen Transport Temperature

Ambient

Additional Information

For BJH Laboratory Use Only

Analyte Stability:

  • Processed sample stability:
    • Ambient - 2 days
    • Refrigerator (2-8°C) - 7 days
    • Frozen (≤-20°C) - 7 days
  • See Analyte Stabiltiy Chart under General Information for a complete list.


Laboratory Processing Instructions:

Test performed in BJH Core Lab.  Processed by automated line for delivery to the Cobas 8000. Completed specimens are stored refrigerated for 5 days. 

Special Instructions

LOINC Code Information

Name LOINC Code
Methadone, Urine 70149-0