Test Code 4732-SA HLA: Single Antigen Antibody Screen, Blood
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Methodology
Flow Cytometry Based
Note: HLA antibody identification may be performed as Class ISA ID only, Class IISA ID only, or Class I and II.
Specimen Requirements
Specimen must arrive within 24 hours of draw.
Specimen Type: Whole blood
Container/Tube: 10 mL Red/gray top (SST)
Specimen Volume: ADULT/CHILDREN -Full tube (10 mL) INFANT- 2-3 mL minimum
Additional Information:
1. Do not refrigerate.
2. Renal dialysis patients should always be drawn before dialysis procedure.
Day(s) Test Set Up
Monday through Friday
Turnaround Time:
STAT: 48 hours. (Call HLA Laboratory at 314-362-5322 to request STAT)
Routine: 1 week
Test Classification and CPT Coding
86832
86833
Reference Values
Normal: negative
A positive result indicates the presence of IgG anti-HLA antibodies to either Class I or Class II antigens.
Results are reported as percent positive reactions.
Antibodies identified are reported.
Specimen Transport Temperature
Ambient/Refrigerate NO/Frozen NO
Additional Information
For BJH Laboratory Use Only
Alternate Tube:
Red top tubes
Gold top tubes (SST)
Laboratory Processing Instructions:
Test performed in BJH HLA Laboratory.