Test Code CY012 Cytology, Anal-Rectal Pap Test
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Methodology
Barnes-Jewish Hospital offers ThinPrep Pap test and SurePath Pap test.
Note: Human Papillomavirus (HPV) detection may only be performed on ThinPrep Pap test specimens and at the request of the clinician, at an additional charge.
Specimen Requirements
Specimen Type: Anal-rectal
Container/Tube: ThinPrep or SurePath vial
Specimen Volume: Entire specimen
Collection Instructions:
1. Moisten plastic shaft Dacron swab with tap water.
2. Insert swab about 5 cm to 6 cm into anal canal past anal verge, into rectal vault. This is done without direct visualization of anal canal.
3. Apply firm lateral pressure to swab handle as it is rotated and slowly withdrawn from anal canal, inscribing a cone-shaped arc. Care should be taken to ensure that the transition zone is sampled. Avoid using cotton swabs or wooden stick because handle may break and splinter during collection.
4. Place swab in preservative (ThinPrep or SurePath) vial
and agitate vigorously several times to release cellular
harvest.
5. Label vial with patient’s first and last name, date of
birth, and date of collection.
6. Ensure vial is tightly closed and place in a biohazard
Ziplock bag.
Additional Information: Indicate on requisition (anal cytology specimen).
Forms: Cytology Laboratory Requisition in Special Instructions.
Day(s) Test Set Up
Monday through Friday
Cut-off time: Monday through Thursday 1530; Friday 1600
Turnaround Time:
STAT: not available
Routine: 10 working days
Test Classification and CPT Coding
88112-Cyto-cellular enhancement, liquid based
87624-HPV amplified probe (if appropriate)
Note: Professional charges will be
applied.
Reference Values
An interpretive report will be provided.
Specimen Transport Temperature
Ambient/Refrigerate NO/Frozen NO
Additional Information
For BJH Laboratory Use Only
Laboratory Processing Instructions:
Cytology performed in BJH Cytology. BJH Cytology will forward HPV detection to the performing laboratory, if applicable.