Test Code LAB 274 Direct Antiglobulin Test (Polyspecific), Blood
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Methodology
Testing Algorithm:
If this test is positive, then #9803 Direct Antiglobulin Profile, Blood will be performed, at an additional charge.
If IgG (included in #9803) is positive, then an elution and antibody identification will be performed, at an additional charge.
Specimen Requirements
Hospital Patient:
Specimen Type: Whole blood
Container/Tube: Pink top (EDTA)-Separator gel tube is not acceptable.
Specimen Volume: Full tube
Specimen Minimum Volume: 10 mL (child: 4 mL)
Additional Information:
1. Specimen must be labeled with barcode label or addressograph imprint.
2. Specimen must have either a PPID label, chart or non- computer generated label with one full signature (phlebotomist). Check Sample may be requested on patients without prior ABO/Rh history
Non Hospital Patient:
Specimen Type: Whole blood
Container/Tube: Pink top (EDTA)-Separator gel tube is not acceptable.
Specimen Volume: Full tube
Specimen Minimum Volume: 10 mL (child: 4 mL)
Additional Information:
1. Label tube with patient’s name, date of birth, and Social Security number.
2. Specimen submitted for ABO/Rh only must be signed by person drawing specimen. Initials are not acceptable.
3. If a type and screen is also requested or is added to this request the specimen must have one full signature (phlebotomist). Initials are not acceptable. Check Sample may be requested on patients without prior ABO/Rh history.
Day(s) Test Set Up
Monday through Sunday
Turnaround Time:
STAT: 1 hour after receipt in laboratory
Routine: 2 to 3 hours after receipt in laboratory
Test Classification and CPT Coding
86880-Direct coombs
86880 x 2-Direct coombs (if appropriate)
86860-Antibody elution (if appropriate)
86870-Antibody identification (if appropriate)
Reference Values
Not applicable
Specimen Transport Temperature
Ambient
Additional Information
For BJH Laboratory Use Only
Alternate Tube:
2 red-top tubes
Laboratory Processing Instructions:
Test performed in BJH Blood Bank. Centrifuge upon receipt. After testing is performed, store specimen refrigerated at 2° C to 8° C.