Test Code LAB258 Cryptosporidium and Giardia Antigen Assay
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Methodology
Immunoassay for Giardia lamblia and Cryptosporidium species only.
This test is more sensitive than the microscopic exam for these 2 parasites. If a complete microscopic exam is needed for those patients in whom the likelihood of a parasitic infection other than Giardia lamblia or Cryptosporidium species is higher, please order Complete Microscopic Ova and Parasite Exam on request form (see LAB955 - Parasites, Complete Microscopic Examination, Stool).
Specimen Requirements
Acceptable Specimens:
1. Stool in 1 vial 10% formalin. Stool should be added to the vial to bring liquid level up to “Fill to Here” line; approximately 5 mL of stool.
2. Fresh stool: 10 mL in leak-proof, screw-capped, sterile container
Collection Procedure:
Note: Do not collect stool if the patient has been:
- On the following drugs or using these preparations for the preceding 7 to 10 days: aluminum-containing compounds, barium, bismuth, clindamycin, iron salts, kaolin drugs, magnesium, metronidazole, tetracycline, or trimethoprim/sulfisoxazole.
- Using these preparations for the preceding 2 days: oil laxatives or enemas.
Collect 3 specimens over a 3 to 9 day period for outpatients (do not collect more than 1 per day) or 1 specimen per day for the first 3 days after admission for hospitalized patients. (Specimen collected more than 3 days after a patient is admitted is not acceptable, without consultation. Contact the lab medicine resident: 747-1320 (#3). Virtually all parasite infections are acquired outside the hospital.
Parasitology Transport Kit
Collect stool as described in instructions provided with each kit. (Swab or specimen contaminated with urine or water is not acceptable.)
Fresh stool
1. Place specimen in leak-proof, screw-capped, sterile container.
2. Maintain sterility and forward promptly.
3. If transport is delayed, keep specimen at room temperature. Specimen must be received within 24 hours of collection.
Note: Specimen source is required.
Day(s) Test Set Up
Monday through Friday
Turnaround Time:
STAT: not available
Routine: results available within 24 hours for specimens received Monday through Friday and on Monday for specimens received Saturday and Sunday.
Test Classification and CPT Coding
87328
87329
Reference Values
Negative
Literature Reference:
Garcia Lynne S. Diagnostic medical parasitology. 6th ed. Washington, DC: ASM Press; 2016; 6-25.
Remel Xpect Giardia/Cryptosporidium Package Insert; May 2011
Specimen Transport Temperature
Ambient
Additional Information
For BJH Laboratory Use Only
Minimum Volume:
Transport Kit: Stool should be added to each vial to bring liquid level up to “Fill to Here” line; approximately 5 mL of stool for each vial.
Fresh stool: 10 mL in leak-proof, screw-capped, sterile container.
Laboratory Processing Instructions:
Test performed in BJH Microbiology.