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HelpTest Code LAB2900 Hemoglobin Analysis, Blood
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Methodology
Capillary Electrophoresis/Gel Electrophoresis
Testing Algorithm:
On all first time patients, for hemoglobins that appear in the S or C position, confirmatory testing by acid gel electrophoresis will be performed, at an additional charge. For all other hemoglobins, confirmation testing by acid and alkaline gel electrophoresis will be performed, at an additional charge.
Specimen Requirements
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: Full tube
Day(s) Test Set Up
Tuesday and Thursday
Turnaround Time:
STAT: not available
Routine: Normal findings are resulted Tuesday and Thursday; abnormal finding confirmation results available once signed by medical director-normally by 1530 Friday.
Test Classification and CPT Coding
83020-Capillary electrophoresis
83020-Acid hemoglobin electrophoresis (if appropriate)
83020-Alkaline hemoglobin electrophoresis (if appropriate)
Reference Values
Hemoglobin A1: 96.0%-98.5%
Hemoglobin A2: 1.5%-3.2%
Hemoglobin F: ≤1.0%
Literature References:
1. Hoyer JD, Kroft SH. Color atlas hemoglobin disorders: a compendium based on proficiency testing. College of American Pathologist, 2003.
2. Giordano, PC. Carrier diagnostics and prevention of hemoglobinopathies using capillary electrophoresis. Sebia Laboratory, 2007.
Specimen Transport Temperature
Ambient
Additional Information
For BJH Laboratory Use Only
Analyte Stability:
7 days refrigerated
Laboratory Processing Instructions:
Test performed in BJH Core Lab. RBC profile completed on XN. Refrigerate specimen until assayed on Capillarys..
LOINC Code Information
| Name | LOINC Code |
| Acid Hemoglobin Electrophoresis | 13515-2 |
| Alkaline Hemoglobin Electrophoresis | 49323-9 |
| Hemoglobin A | 20572-4 |
| Hemoglobin A2 | 27345-8 |
| Hemoglobin C | 32681-9 |
| Hemoglobin F | 32682-7 |
| Hemoglobin S | 32683-5 |
| Hemoglobin Analysis Interpretation | 20492-5 |