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Test Code LAB29247 HIT Antibodies w/Reflex to Serotonin Release Assay (SRA)

Performing Laboratory

Barnes-Jewish Hospital Laboratory

 

Reflex Information:

The first positive result during a single hospitalization will be reflexed to Serotonin release Assay (SRA) and sent to the Blood Center of Wisconsin at an additional cost.                

Methodology

Latex enhanced immunoassay

 

Methodology Literature References:

 HemosIL HIT-Ab(PF4-H) package insert issued 10/2016, Instrumentation Laboratory        

Specimen Requirements

Specimen Type: Plasma

Container/Tube: Light-blue top (citrate)

Specimen Volume: Full tube

Specimen minimum volume:  Full tube

 

Collection instructions:

1. Draw a discard tube prior to light-blue top (citrate) tube.
2. Tube should remain stoppered.
3. Do not centrifuge.
4. Clotted blood or an insufficient specimen is not acceptable.
5. Avoid hemolysis and heparin contamination of specimen.

Day(s) Test Set Up

Monday through Sunday

Turnaround Time:
Batched test. Samples received in laboratory by 1 p.m. on each day will be tested and resulted the same afternoon. Samples received in laboratory after 1 p.m. will be tested at 8 a.m. the next day.

 

Test Classification and CPT Coding

86022

Reference Values

Reference Intervals:

Negative      LIA units < 1.0    

 

Reference Interval Literature References

     Theodore E. Warkentin, Jo-Ann I. sheppard, Lori-Ann Linkins, Donald M. Arnold, Ishac Nazy Performance characteristics of an automated latex immunoturbidimetric assay [HemosIL HIT-Ab (PF4)] for the diagnosis of immune heparin-induced thrombocytopenia. Thrombosis Research 153 (2017) 108-117.

Additional Information

For BJH Laboratory Use Only
Analyte Stability:

8 hours at 2° C to 24° C.
Platelet-free plasma can be stored 6 months at -70° C.

Laboratory Processing Instructions:

Test performed in BJH Core Lab.

LOINC Code Inofrmation

Name LOINC Code
HIT Antibodies w/Reflex to Serotonin Release Assay (SRA)

57761-9