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Test Code LAB306 Factor VIII, Plasma

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Absorbance measurement, Clot signature formation

Literature References:

HemosIL SynthASil (0020006800) package insert issued 06/2017, Instrumentation Laboratory.

Specimen Requirements

Specimen Type: Plasma

Container/Tube: Light-blue top (citrate)

Specimen Volume:  Full tube

Specimen Minimum Volume:  Full tube

Collection Instructions:

  1. Draw a discard blue top tube prior to drawing a light-blue top (citrated) tube if using a Winged Infusion Set.  Discard tube should be under filled and discarded.
  2. Clotted blood or an insufficient specimen is not acceptable.
  3. Avoid phlebotomy techniques which cause hemolysis and heparin contamination.
  4. Follow nursing policy on volume of blood to discard prior to collecting blood from indwelling heparinized catheters.

Analyte Stability:

4 hours at 18° C to 24° C.

Specimen can not be refrigerated.

Transported frozen -20° C:  When Factor VIII activity is performed on frozen plasma, the result obtained may be up to 33% lower compared to the result obtained on fresh plasma.

 

Additional Information: If the patient is a hospital patient and the hematocrit is >55%, call Barnes-Jewish Hematology Laboratory at 314-362-5203 for instructions.

Day(s) Test Set Up

Monday through Sunday
Turnaround Time:
STAT: 2 hours after receipt in laboratory
Routine: 2-4 hours after receipt in the laboratory

Test Classification and CPT Coding

85240

Reference Values

45%-160%

Literature References:

1. Colman RW, Hirsch J, Marder VJ, Salzman EW. Hemostasis and thrombosis, basic principles and clinical practice. Philadelphia:  J.B. Lippincott Co., 1982.
2. Thompson AR, Harke LA. Manual of hemostasis and thrombosis, Edition 3. Philadelphia:  F.A. Davis Co., 1983.

Specimen Transport Temperature

Ambient Yes/Refrigerate NO/Frozen Yes, see disclaimer

Disclaimer: Frozen -20° C:  When Factor VIII activity is performed on frozen plasma, the result obtained may be up to 33% lower compared to the result obtained on fresh plasma.

Additional Information

For BJH Laboratory Use Only
Analyte Stability:

4 hours at 18° C to 24° C.

Specimen cannot be refrigerated.

Laboratory Processing Instructions:

Test performed in BJH Core Lab.

LOINC Code Information

Name LOINC Code
Factor VIII, Plasma 3208-6