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Test Code LAB3061 Complete Blood Count (CBC) with Differential, Blood

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Sysmex XN (Electrical Impedance)

Includes WBC, RBC, hemoglobin, hematocrit, RBC indices, RDW-CV, RDW-SD, platelet, MPV, nRBC (percent and absolute) and WBC differential (percent and absolute).

 

If an abnormal value or flag is triggered on the initial patient specimen, a manual smear will always be reviewed. Any subsequent specimen within 72 hours will be verified by the “Consistent Rule” unless there is a change in the values and flags in which case a new smear review will be conducted. 

Literature References:

Resource Manual:  Sysmex XN-Series Automated Hematology Systems, Document Number:  1002-LSS, Rev. 2, January 2013

Specimen Requirements

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: Full tube

Collection Instructions: Immediately invert several times to mix blood.

Day(s) Test Set Up

Monday through Sunday

Turnaround Time:
STAT: 1 hour after receipt in laboratory 
Routine: 2-4 hours after receipt in laboratory

Test Classification and CPT Coding

85025

See additional CPT 85055 Immature Platelet Fraction (IPF) if PLT-F <50 K/cumm.

 

Reference Values

WBC

3.8-9.9 K/cumm
 

Physician alert values:

Inpatients: <1.0 K/cumm or >50.0 K/cumm.  Inpatient alert values are called the first time and if consistent, resulted as “Consistent with previous” for 30 days.

Outpatients: <1.0 K/cumm or >50.0 K/cumm.  Outpatient alerts are called each time.


RBC
Males
4.3-5.8 M/cumm
Females
3.9-5.2 M/cumm


HEMOGLOBIN
Males
13.0-17.5 g/dL
Females
11.9-15.5 g/dL
 

 Physician alert values:

Inpatients:  < 6.5 g/dL and >/= 20.0 g/dL.  Inpatient alert values are called the first time and if consistent, resulted as “Consistent with previous” for 72 hours.

Outpatients: <6.5 g/dL and >/= 20.0 g/dL.  Outpatient alerts are called each time.

 

HEMATOCRIT
Males
38.9%-50.3%
Females
35.6%-45.5%

 

MCV

81.3-96.4 fl

 

MCH

27.1-33.3 pg

 

MCHC

32.3-35.7 g/dL

 

RDW-CV

11.1%-14.9%

 

RDW-SD

35.7-48.1 fL

 

PLATELETS
150-400 K/cumm
 

Physician alert values:

Bone Marrow Transplant Inpatients: < 10 K/cumm.  BMT alert platelet counts are called each time.

Non-BMT Inpatients: <20 K/cumm and >1,000 K/cumm. 

Inpatient alert values < 20 K/cumm are called each time. 

Inpatient platelet counts > 1,000 K/cumm are called the first time and if consistent, resulted as “Consistent with previous” for 30 days.

Outpatients: <50 K/cumm and >1,000 K/cumm. Outpatient alerts are called each time.


 

MPV

9.1-12.3 fl

 

IMMATURE PLATELET FRACTION %

1.6-10.1%

 

ABSOLUTE NEUTROPHIL

1.5-6.5 K/cumm

Physician alert value: <0.5 K/cumm. 

Inpatient and outpatients are called each time. 

 

ABSOLUTE LYMPHOCYTE

0.8-3.3 K/cumm

 

ABSOLUTE MONOCYTE

0.2-0.8 K/cumm

 

ABSOLUTE EOSINOPHIL

0-0.5 K/cumm

 

ABSOLUTE BASOPHIL

0-0.1 K/cumm

 

ABSOLUTE IG

0.0-0.1 K/cumm

 

nRBC Absolute

0.00-0.01

 

BLAST

0.0%

Physician alert value: 1%. 

Inpatient are called the first time and if consistent, resulted as “Consistent with previous” for 30 days.

Outpatients are called each time. 

 

Literature References:

1. Henry JB. Clinical diagnosis and management. WB Saunders Co.,1979.

2. McKenzie S. Clinical laboratory hematology. Pearson Prentice Hall, 2004:5.

3. Medicine Net. Complete blood count, medicinenet.com/complete 1996-2006.

Specimen Transport Temperature

Ambient

Additional Information

For BJH Laboratory Use Only
Alternate Tube:

If pink-top (EDTA) tube is available, sample must be front loaded and approved for use by supervisor.

Analyte Stability:

Refrigerated: up to 24 hours

Laboratory Processing Instructions:
Test performed in BJH Core Lab

LOINC Code Information

Name LOINC Code
WBC 6690-2
RBC 789-8
Hemoglobin 718-7
Hematocrit 4544.3
MCV 787-2
MCH 785-6
MCHC 786-4
RDW-SD 21000-5
RDW-CV 788-0
Platelets 777-3
MPV 32623-1
Neutrophil 770-8
Lymphocytes 736-9
Monocytes 5905-5
Eosinophil 713-8
Basophil 706-2
Immature Granulocytes 71695-1
Absolute IG 53115-2
Absolute Neutrophil 751-8
Absolute Lymphocyte 731-0
Absolute Monocyte 742-7
Absolute Eosinophil 711-2
Absolute Basophil 704-7
NRBC % 58413-6
NRBC # 771-6
Immature Platelet Fraction % 71693-6