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Test Code LAB4512 Lymphocyte Subpop 10

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Profile Information:
CD3 TCR-Alpha/Beta
CD4 HLA-DR
CD8 CD3 HLA-DR
CD4/CD8 Ratio CD4 CD45 RA
CD16+CD56 CD4 CD45 RO
CD19 CD8 CD45 RA
Double Negative T-Cells (DNTs) CD8 CD45 RO

Method Name:

Flow Cytometry

Literature References:

1. U.S. Department of Health and Human Services, Public Health Service, CDC. Flow cytometric immunophenotyping quality assurance and quality procedures for CD4+ T-Lymphocyte Determinations. April 1993.
2. NCCLS Tentative Guidelines. Clinical application of flow cytometry: Quality assurance and immunophenotyping of peripheral blood lymphocytes. H42T 12:6.

Specimen Requirements

Specimen must arrive within 24 hours of draw. Specimen must arrive by 1400 on Friday and 1 day before a holiday.

 

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: Full tube

Specimen Minimum Volume: 1.5 mL

Day(s) Test Set Up

Monday through Friday
Cut-off time: 2100 Monday through Thursday; 1600 Friday and eve before a holiday
Turnaround Time:
STAT: not available
Routine: 48 hours

Test Classification and CPT Coding

86355-B-cells, total count

86357 -Natural killer cells, total count
86359-T-cells, total count
86360-T-cells CD4+CD8 with ratio
86356 x 8-Mononuclear cell antigen, quantitative

Reference Values

Total HLA-DR
No established reference intervals

 

CD3 HLA-DR
No established reference intervals


TCR ALPHA/BETA
No established reference intervals

 

DOUBLE NEGATIVE T-CELLS (DNTS)
No established reference intervals

 

CD4/CD8 RATIO

0.9-4.4

 

 

  Total Cells
 (CD3)		 Helper Cells
 (CD4)		 Cytotoxic
T-Cells (CD8)		 B Cells
(CD19)		 Natural Killer
CD16+/CD56+
0-3 Months          
Percentage: 53%-84% 35%-64% 12%-28 6%-32% 4%-18%
Absolute (Cells/mcL): 2500-5500 1600-4000 560-1700 300-2000 170-1100
3-6 Months          
Percentage: 51%-77% 35%-56% 12%-23% 11%-41% 3%-14%
Absolute (Cells/mcL): 2500-5500 1800-4000 590-1600 430-3000 170-830
6-12 Months          
Percentage: 49%-76% 31%-56% 12%-24% 14%-37% 3%-15%
Absolute (Cells/mcL): 1900-5900 1400-4300 500-1700 610-2600 160-950
1-2 Years          
Percentage: 53%-75% 32%-51% 14%-30% 16%-35% 3%-15%
Absolute (Cells/mcL): 2100-6200 1300-3400 620-2000 720-2600 180-920
2-6 Years          
Percentage: 53%-75% 28%-47% 16%-30% 14%-33% 4%-17%
Absolute (Cells/mcL): 1400-3700 700-2200 490-1300 370-1400 130-720
6-12 Years          
Percentage: 60%-76% 31%-47% 18%-35% 13%-27% 4%-17%
Absolute (Cells/mcL): 1200-2600 650-1500 370-1100 270-860 100-480
12-17 Years          
Percentage: 56%-84% 31%-52% 18%-35% 6%-23% 3%-22%
Absolute (Cells/mcL): 1000-2200 530-1300 330-920 110-570 67-1134
Adult          
Percentage: 60%-88% 31%-64% 12%-40% 6%-25% 5%-25%
Absolute (Cells/mcL): 661-1963 365-1294 187-781 86-488 76-467

 

CD45 RO & RA

Pediatric Reference Values (2-18y)

Cell Subset

Reference Range Percentage

Reference Range Absolute (cells/mcL)

CD4+CD45RA+

21-75

135-893

CD8+CD45RA+

23-88

83-653

CD4+CD45RO+

11-44

56-411

CD8+CD45RO+

2-26

10-142

 

Adult Reference Values (19-84y)

Cell Subset

Reference Range Percentage

Reference Range Absolute (cells/mcL)

CD4+CD45RA+

3-59

27-833

CD8+CD45RA+

6-84

19-508

CD4+CD45RO+

15-69

167-670

CD8+CD45RO+

4-49

15-275

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen NO

Additional Information

For BJH Laboratory Use Only
Alternate Tube: 

NONE

 

Analyte Stability:

Specimen cannot be refrigerated. Specimen must be processed within 24 hours of draw and kept at ambient temperature.
 

Laboratory Processing Instructions:

Test performed in BJH Flow Cytometry. WBC and lymphocyte percent is needed to calculate absolute values. Run and verify WBC and lymphocyte percent on all specimens prior to giving specimen to Flow Cytometry.