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Test Code LAB5134 Iron Profile, Plasma

Performing Laboratory

Barnes-Jewish Hospital Laboratory

Methodology

Colorimetric (Ferrochrome/Ferrozine)

Literature References:

1. Package insert: Iron IRON2 reagent. Roche Corporation, 07/2019.
2. Package insert: Unsaturated iron-binding capacity (UIBC) reagent. Roche Corporation, 10/2019.

 

Includes iron, iron-binding capacity, and transferrin saturation.

Specimen Requirements

Patient Preparation:

There can be considerable intraindividual variation through the course of a day. A fasting, morning specimen is recommended.

 

Specimen Type: Plasma

Container/Tube: Mint-green top (lithium heparin)
Specimen Volume: Full tube

Day(s) Test Set Up

Monday through Sunday

Turnaround Time:
STAT: 1 hour after receipt in laboratory 
Routine: Routine: 2 to 4 hours after receipt in laboratory

Test Classification and CPT Coding

83540-Iron
83550-Iron-binding capacity

Reference Values

Analyte Age Gender Reference Range
Iron 0 minutes-1 month Female 100-250 mcg/dL
  > 1 month-12 years Female 50-120 mcg/dL
  > 12 - 150 years Female 35-145 mcg/dL
  0 minutes-1 month Male 100-250 mcg/dL
  > 1 month-12 years Male 50-120 mcg/dL
  > 12 - 150 years Male 50-150 mcg/dL
Iron Binding Capacity 0 minutes - 30 days All 90-350 mcg/dL
  > 30 days-150 years All 250-400 mcg/dL
Transferrin Saturation 0 minutes - 18 years All 10-45%
  > 18 years-150 years All 20-50%

Specimen Transport Temperature

Ambient <6 hours/Refrigerate <24 hours OK

Additional Information

For BJH Laboratory Use Only
Alternate Tube:

1 dark green-top (lithium heparin) tube
1 red-top tube

Laboratory Processing Instructions:

Test performed in BJH Core Lab.  Processed by automated line for delivery to the Cobas 8000.  Completed specimens are stored refrigerated 5 days.

Test performed in Ambulatory Cancer Building Lab.  Centrifuge sample and deliver to the Cobas.  Completed specimens are stored refrigerated for 5 days. 

Special Instructions

See Analyte Stability Chart under General Information for a complete list.

LOINC Code Information

Name LOINC Code
Iron 2498-4
Iron/Iron binding capacity, total 2505-6
Iron saturation 2502-3