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Test Code LAB5286 Lactate/Pyruvate Panel, Blood2

Important Note

Call Barnes-Jewish Hospital Laboratory Customer Service at 314-362-1470 to obtain a perchloric acid (PCA) tube and to schedule a STAT pick-up.

Core Lab Note: Please verify that order is not for Lactate Rapid Whole Blood, for which results are available STAT.

Performing Laboratory

St. Louis Children's Hospital Laboratory

Methodology

Methodology: Enzymatic

Specimen Requirements

Specimen Type: Blood
Container/Tube Preferred/Acceptable: Perchloric acid (PCA) Vacutainer. Call Laboratory Customer Service  at 314-362-1470 for special PCA collection tube if needed
Collection Volume: 1.0 mL
NOTE: minimum of 0.5 mL whole blood may be used but MUST call lab to get an adjusted PCA tube for reduced volume. 
Patient Preparation: Remove tourniquet from patient's arm before collecting specimen.
Sample Rejection:

Specimens collected in other than PCA tubes.
Specimens with incorrect volume of blood to PCA ratio.
Specimens that are grossly hemolyzed.
Mislabeled or unlabeled specimens. 
Special Instructions:

1. Call Laboratory Customer Service at 314-362-1470 to have PCA tube sent for collection.
2. Collect exactly 1.0 mL of whole blood in a syringe
3. Remove needle from syringe and remove top of PCA tube. Gently push blood from syringe into PCA tube. Place top back on PCA tube and mix tube vigorously to fully deproteinize the sample.
4. Forward to lab promptly.

For outside facilities sending to lab: if specimen cannot be received at BJH Core Lab within 6 hours, refrigerate aliquot and send on wet ice.

Day(s) Test Set Up

Day(s) Test Set Up: Monday-Sunday
Turnaround Time: 1-3 days; results available day of testing
Lab Testing Section: Chemistry

Reference Values

Reference Range:

Lactate:  0.5-2.0 mmol/L
Pyruvate:  0.03-0.10 mmol/L
Critical Values

Lactate:
Critical value (automatic callback): >8.0 mmol/L
Critical test results will be called initially and every 24 hours thereafter.
 Limitations: This assay does not measure D(-) Lactate. The enzyme used is L (+) lactate dehydrogenase.

Additional Information

For SLCH Laboratory use only

Lab Processing instructions: Centrifuge specimen to separate and aliquot into a conical tube. Refrigerate specimen if received after cut-off time.

For outside facilties sending to lab: Label aliquot as "PCA aliquot". If specimen cannot be received at SLCH lab within 6 hours, refrigerate aliquot and send on wet ice.
Processed Volume: 

Remove all of the supernatant without getting any of the cellular components.
A minimum of 0.3 mL supernatant should be removed and placed in conical tube.
Analyte Stability:

  LACTATE PYRUVATE
Ambient: NA NA
Refrigerated 8 days (supernatant) 2 days (supernatant)
Frozen NA DO NOT freeze

NOTES: 

1.  Specimen may also be collected with 0.5 mL of whole blood but MUST be added to and mixed in an adjusted PCA tube with only 1.0 mL of perchloric acid.
2.  Reject due to gross hemolysis.
3.  Reject if specimen to PCA ratio is not correct.

Additional Test Codes

EPIC order name: Lactate and Pyruvate (EPIC Test# LAB5286)
Cerner order name: Lact/Pyruv Panel
CPT Codes:

83605: Lactate
84210: Pyruvate
LOINC Value

Lactate: 2524-7
Pyruvate: 14121-8