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HelpTest Code LAB5669 AIDS Clinical Trial Unit (ACTU)
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Methodology
| Profile Information: | |
| CD3 | |
| CD3 Absolute | |
| CD4 | |
| CD4 Absolute | |
| CD4/CD8 Ratio | |
| CD8 | |
| CD8 Absolute | |
Method Name:
Flow Cytometry-Single Platform
Literature References:
1. U.S. Department of Health and Human Services, Public Health Service, CDC. Flow cytometric immunophenotyping quality assurance and quality procedures for CD4+T-lymphocyte determinations. 04/1993.
2. NCCLS tentative guidelines. Clinical application of flow cytometry: quality assurance and immunophenotyping of peripheral blood lymphocytes. H42T 12:6.
3. MMWR Recommendation and Reports, Guidelines for performing single-platform absolute CD4 determination with CD45 gating for persons infected human immunodeficiency virus, January 21, 2003.
Specimen Requirements
Specimen must arrive within 24 hours of draw. Specimen must arrive by 1600 on Friday and 1 day before a holiday.
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: Full tube
Specimen Minimum Volume: 1 mL
Additional Information: Specimen cannot be refrigerated.
Day(s) Test Set Up
Monday through Friday
Cut-off time: 2100 Monday through Thursday; 1600 Friday and eve before a holiday
Turnaround Time:
STAT: not available
Routine: 48 hours
Test Classification and CPT Coding
86359-T-cells, total count
86360-T-cells CD4+CD8 with ratio
Reference Values
CD4/CD8 RATIO
0.9-4.4
| Total Cells (CD3) |
Helper Cells (CD4) |
Cytotoxic T-Cells (CD8) |
|
| 0-3 Months | |||
| Percentage: | 53%-84% | 35%-64% | 12%-28 |
| Absolute (Cells/mcL): | 2500-5500 | 1600-4000 | 560-1700 |
| 3-6 Months | |||
| Percentage: | 51%-77% | 35%-56% | 12%-23% |
| Absolute (Cells/mcL): | 2500-5500 | 1800-4000 | 590-1600 |
| 6-12 Months | |||
| Percentage: | 49%-76% | 31%-56% | 12%-24% |
| Absolute (Cells/mcL): | 1900-5900 | 1400-4300 | 500-1700 |
| 1-2 Years | |||
| Percentage: | 53%-75% | 32%-51% | 14%-30% |
| Absolute (Cells/mcL): | 2100-6200 | 1300-3400 | 620-2000 |
| 2-6 Years | |||
| Percentage: | 53%-75% | 28%-47% | 16%-30% |
| Absolute (Cells/mcL): | 1400-3700 | 700-2200 | 490-1300 |
| 6-12 Years | |||
| Percentage: | 60%-76% | 31%-47% | 18%-35% |
| Absolute (Cells/mcL): | 1200-2600 | 650-1500 | 370-1100 |
| 12-17 Years | |||
| Percentage: | 56%-84% | 31%-52% | 18%-35% |
| Absolute (Cells/mcL): | 1000-2200 | 530-1300 | 330-920 |
| Adult | |||
| Percentage: | 60%-88% | 31%-64% | 12%-40% |
| Absolute (Cells/mcL): | 661-1963 | 365-1294 | 187-781 |
Specimen Transport Temperature
Ambient/Refrigerate NO/Frozen NO
Additional Information
For BJH Laboratory Use Only
Alternate Tube:
1 dark green-top (sodium heparin) tube
Analyte Stability:
Specimen cannot be refrigerated. Specimen must be processed within 72 hours of draw and kept at ambient temperature.
Laboratory Processing Instructions:
Test performed in BJH Flow Cytometry.