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Test Code LAB5793 Ehrlichia/Anaplasma Detection by PCR

Performing Laboratory

 

Barnes-Jewish Hospital Molecular Infectious Disease Laboratory   

Methodology

Polymerase Chain Reaction (PCR) performed on the DiaSorin Liaison MDX.         

Specimen Requirements

Acceptable Specimens

Blood

1. Draw blood into Lavender-top/EDTA tube. Avoid hemolysis.

2. Maintain sterility and forward promptly at ambient temperature only.

3. Whole blood is required for testing. (Plasma or serum is not acceptable.)

Day(s) Test Set Up

Monday through Sunday

Turnaround Time:
STAT: not available
Routine: 1- 3 days, Final report available the day of testing                     

Test Classification and CPT Coding

Test classification: 

This test has been evaluated for the detection of Anaplasma phagocytophilum, Ehrlichia chafeensis, Ehrlichia ewingii, and Ehrlichia canis. 

This assay utilizes ASR’s (analyte specific reagents) and is not FDA cleared.  This test was developed and its performance has been evaluated by the Barnes Jewish Molecular Infectious Disease Laboratory for performance on whole blood specimens.

Assay limit of detection:

500 copies/mL in whole blood for Ehrlichia spp.

250 copies/mL in whole blood for Anaplasma phagocytophilum.

 

Limitations:

-The detection of target nucleic acid is dependent upon proper sample collection, transport, handling and storage. Failure to observe proper procedures in any one of these steps can lead to incorrect results.

-False-negative results may occur if the target nucleic acid is present at a level that is below the analytical sensitivity of the assay or if there are genomic mutations, insertions, deletions, or rearrangements or if performed very early in the course of illness.

 

CPT Code:

87798 x 2

 

Reference Values

Negative

Additional Information

For BJH Laboratory Use Only

 

Minimum Volume: 
Blood: 1 mL of EDTA

 Laboratory Processing Instructions:    

 BJH Microbiology will forward to the performing laboratory.  

Special Instructions

Specimen Transport Instructions

Blood-Ambient/Refrigerate/Frozen OK

Refrigerated specimens must be tested within 7 days
Frozen specimens must be tested within 30 days