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Test Code LAB6644 Vasculitis Antibody Screen with reflex to ANCA

Performing Laboratory

Barnes Jewish Hospital Laboratory

Methodology

Multiplex Flow Immunoassay

Testing Algorithm:

If MPO or PR3 are ≥1 AI then Antineutrophil cytoplasmic antibodies (ANCA) will be performed at an extra charge.

Literature references:

  1. Bossuyt X et al. Revised 2017 international consensus on testing of ANCAs in granulomatosis with polyangiitis and microscopic polyangiitis. Nature Reviews Rheumatology2017;13:683-92.
  2. Package Insert: BioPlex 2200 Vasculitis kit. BioRad Laboratories, Inc. 04/2012

Specimen Requirements

Specimen Type: Serum

Container/Tube: Plain red top

Specimen Volume: Full tube

Day(s) Test Set Up

Monday through Sunday

Cut-off time: 1000

Turnaround Time:
STAT: not available
Routine: results available the day of testing

Test Classification and CPT Coding

83876-MPO 

83520-PR3 

86036-ANCA (if appropriate)

86037-ANCA Titer (if appropriate)

Reference Values

MPO and PR3

Negative: < 1.0 AI

Positive: > 1.0 AI

Literature Reference:

Package Insert: BioPlex 2200 Vasculitis kit. BioRad Laboratories, Inc. 04/2012

Specimen Transport Temperature

Ambient

Additional Information

For BJH Laboratory Use Only
Laboratory Processing Instructions:

Test performed in BJH Core Lab. Processed by automated line and aliquot stored refrigerated for batch analysis. Completed specimens are stored refrigerated for 5 days.

LOINC Code Information

Name

LOINC Code

Myeloperoxidase Ab

29643-4

Proteinase 3 Ab

29644-2

Neutrophil Cytoplasmic Ab, Qual

17351-8